Ryan Chaparian

4 Questions 17 Answers 0 Followers

Questions related from Ryan Chaparian

Transduced cells survive selection but fail to express GOI?

I'm trying to make a stable HEK293T cell line that expresses GFP only in the presence of a specific unnatural amino acid (UAA). I packaged lentivirus particles containing my expression cassette,...

04 April 2022 9,986 11 View

Can 2D-PAGE be used to determine structural changes in nucleoprotein complexes?

Hi, I'm currently working on an experiment in which I show competitive DNA binding between 2 proteins via an electrophoretic shift mobility assay (EMSA, see attached image). I have been able that...

29 May 2019 337 0 View

A very odd Western blot transfer issue...?

Hi all, I am having a very odd issue regarding my Western blot transfers. When I perform a transfer, sometimes my bands diffuse across huge areas of the membrane and become 'wave-like.' I've...

15 January 2018 3,088 10 View

in SDS-PAGE, how can I prevent well walls from collapsing?

I use the CBS Scientific gel assembly apparatus (http://www.cbsscientific.com/verticalmini-gelelectrophoresissystemaccessories.aspx) to make polyacrylamide gels. Lately, I have been experiencing a...

16 January 2015 5,954 6 View