I know so far that it can affect GFP to not be completely uncoupled from the membrane protein and could ruin molecular weight on SDS-PAGE as well as protein function
Dear all I have tried 1ml Trizol to extract total RNA from crabs gills. The weight of most of my samples is + -0,03 g. We have applied this protocol for hepatopancreas samples and the results...
03 June 2019 1,447 8 View
Hi, I found these little circle caps/membranes(?) in my lab, they are stored in an odourless liquid that is not water (I tried mixing some in and could see the fluids mixing). I work in a...
19 February 2019 7,404 7 View
I wanted to know if enrichment kits can also be used for cell lines or if they can only be used for buffy coat and peripheral blood samples? Thank you!
28 January 2019 7,351 3 View
I am trying to determine the binding between a protein and cofactor, however the KD value cannot be found online/published. Would the concentrations of my receptor I choose for my assay be more of...
10 May 2018 6,029 5 View
I'm a quite new to growing cells and have only worked with HAC15-cell line and H295R-cell line. The HAC15-cells look very different from H295R, and I'm starting to wonder if they are healthy or...
24 September 2017 1,038 1 View
14 July 2016 6,869 3 View
29 January 2015 3,533 7 View
13 August 2014 3,649 1 View
Does anyone have experience with the markers expressed on K562 cells? I have checked DSMZ and when I stain my cells with the markers stated that K562 should be positive for and run the profile on...
01 January 1970 563 3 View
I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance
03 March 2021 3,568 1 View
I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...
01 March 2021 9,310 4 View
Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?
01 March 2021 9,447 3 View
Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...
01 March 2021 2,622 3 View
I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...
28 February 2021 4,521 3 View
I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...
28 February 2021 7,127 2 View
I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...
28 February 2021 7,370 3 View
I think we were all surprised at the first time we got to know quantum mechanics that the squared modulus of the wave function is the probability density of the existence of the particle? The role...
28 February 2021 6,282 9 View
What could be the reason of moisture at the sides of Vial when I am preparing my polmyer+DMSO solution for membrane fabrication? What etiquettes I am possibly missing here?
28 February 2021 6,516 5 View
Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.
27 February 2021 9,356 3 View