FBS,Hydrocortisone, Epidermal Growth Factors(EGF), Insulin, Cholera toxins, and other supplements  are used in establishing cell culture. some protocols used all of them, others used some of them, So I want to know what are the basic components should be found and what I can continue with out using them, particularly  if you know I am working on establishing culture for breast cancer tissue (epithelial and fibroblasts)    

Thanks,

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