Mohamed Farghaly

6 Questions 5 Answers 0 Followers

Questions related from Mohamed Farghaly

What is the source of estrogen in breast cancer culture?

In breast, Estrogen is synthesized from androgens by aromatase enzyme. In breast cancer cell culture, where the adrenal androgenic precursors are not present, what could be the source of estrogen?...

10 May 2017 2,394 6 View

In establishing cell culture, which supplements are essential and can affect the validity of my culture and which ones I can continue if absent ?

FBS,Hydrocortisone, Epidermal Growth Factors(EGF), Insulin, Cholera toxins, and other supplements are used in establishing cell culture. some protocols used all of them, others used some of them,...

18 December 2014 1,473 2 View

For in vitro assessment of breast cancer drugs what is your best choice : cell lines,primary culture or explant cell culture?

There are many ways for assessment of Breast Cancer drugs however every way has limitations : primary culture leads to loss of cell- cell interaction,explant culture had limited success and cell...

01 December 2014 3,840 4 View

How can I measure the effect of aromatase inhibitors in vitro?

Can any one help me in the methods by which I can detect the effect of aromatase inhibitors in treating breast cancer in vitro (Cell culture and induced cancers in mice). Thanks

15 September 2014 7,807 2 View

Breast tissue enzymatic disaggregation : Trypsin or collagenase??

For breast tissue enzymatic disaggregation what do you prefer Trypsin or collagenase and what are precautions I should take in choosing the media and other reagents for every one of them. Thanks

13 September 2014 4,665 2 View

What type of cells produce the enzyme aromatase in breast cancer?

I want to know whether cancerous or normal cells have the greater contribution in the production of aromatase.

14 May 2014 8,975 7 View