We are trying to improve the detection of a synthetic ~3KD hydrophobic peptide in SDS gel electrophoresis. Conditions we already tried are:

1. Using double concentration of SB vs. single recommended.

2. Using High SDS concentration in upper chamber running buffer (0.4 % vs.0.1%)

3. Fixation in formaldehyde-methanol solution.

4. Washing in water after running and after the fixation step.

5. Heating of samples at 70C for 10 minutes.

6. Using simply a blue staining kit (based on water without acetate) vs. coomassie blue staining.

We would appreciate other suggestions.