25 July 2018 3 1K Report

I have a Lenti-FasL plasmid (CMV promoter) I bought from GeneCopoeia, and I am trying to produce Lentiviral particles containing the human FasL plasmid.

For that I am using 293FT cells with Lipofectamine 2000 or LTX.

In all of my attempts, the 293FT cells die after a few hours, probably because they express the FasL gene and kill each-other (they naturally express Fas- receptor).

Does any of you has some experience with making these kind of lenti-particles? any insights?

my plasmid is from Genecopia: http://www.genecopoeia.com/product/search/detail.php?prt=1&cid=&key=L0009

Thank you!

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