Has anyone had any problems with setting the FSC and SSC and getting rid of the background noise with a partec cube 4 colour flow cytometer? Also when we use Percep in the FL3 channel it gives a very weak signal and very poor separation of positive and negative cells.
Reza Habibian
To getting rid of the back ground noise you must set the low and high limits on Flomax software for any parameters. for example you can set the low limit (LL) of FSC and SSC on 100 to delete the back ground noise (check the attached pic). check the FL3 channel with other colors like PI. If the channel detects the signals properly, there is a problem with you cells, conjugated Ab or staining method.
Try the mentioned suggestions and tell us about results.
Good luck.
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