Gathsaurie Malavige

7 Questions 13 Answers 0 Followers

Questions related from Gathsaurie Malavige

Immunohistochemistry of liver sections for oxidative stress?

We want to carry out IHC in paraffin embedded liver tissue for hypoxic damage and oxidative stress. There seems to be so many different types of markers to look at. Does anyone have experience in...

02 November 2016 828 3 View

Does anyone have a protocol for doing platelet activating factor (PAF) from serum?

We did 12 patients and got very high values using a PAF ELISA and then when we did 8 more patients (with same disease severity) using the same PAF ELISA we got much lower values. 1. Is it OK to...

07 February 2014 4,752 1 View

How to do ICS for IL-10 in B cells?

All protocols mention that when you do ICS for IL-10 to identify regulatory B cells that before surface staining you have to block with antibodies for Fc receptors. Can anyone explain why please?

23 January 2014 6,659 3 View

Can anyone help with FSC and SSC using Partec 4 colour flow cytometer?

Has anyone had any problems with setting the FSC and SSC and getting rid of the background noise with a partec cube 4 colour flow cytometer? Also when we use Percep in the FL3 channel it gives a...

03 January 2014 9,083 1 View

Does someone have a protocol to quantify dengue viruses?

Any dengue virus would do, we don't necessarily want to know the serotype. We just want to measure the viral loads in serum in general.

22 November 2013 6,033 2 View

Expression of data as MFI or percentage of positive cells in flow cytometry?

If we are looking at a receptor expression on T cells (IL-10R) is it better to use the % of positive cells, mean fluorescence intensity, or median fluorescent intensity?

22 November 2013 3,085 5 View

Why do we get very hazy plaques in our plaque assay for dengue?

how can we get clearer plaques? we use BHK-21 cell lines.

28 January 2013 6,511 4 View