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Questions related from Gathsaurie Malavige
We want to carry out IHC in paraffin embedded liver tissue for hypoxic damage and oxidative stress. There seems to be so many different types of markers to look at. Does anyone have experience in...
02 November 2016 904 3 View
We are looking at LPS levels in serum in certain infections and as controls we use serum from healthy individuals. All healthy individuals were fasting for 12 hours and blood was separated in...
15 May 2015 1,193 4 View
We did 12 patients and got very high values using a PAF ELISA and then when we did 8 more patients (with same disease severity) using the same PAF ELISA we got much lower values. 1. Is it OK to...
07 February 2014 4,797 1 View
All protocols mention that when you do ICS for IL-10 to identify regulatory B cells that before surface staining you have to block with antibodies for Fc receptors. Can anyone explain why please?
23 January 2014 6,728 3 View
Has anyone had any problems with setting the FSC and SSC and getting rid of the background noise with a partec cube 4 colour flow cytometer? Also when we use Percep in the FL3 channel it gives a...
03 January 2014 9,146 1 View
Any dengue virus would do, we don't necessarily want to know the serotype. We just want to measure the viral loads in serum in general.
22 November 2013 6,097 2 View
If we are looking at a receptor expression on T cells (IL-10R) is it better to use the % of positive cells, mean fluorescence intensity, or median fluorescent intensity?
22 November 2013 3,130 5 View
how can we get clearer plaques? we use BHK-21 cell lines.
28 January 2013 6,562 4 View
