Immortalization of primary mefs using electroporation instead of virus ?

22 January 2017 1 7K Report

Dear all,

Could I use electroporation method to introduce E1A and Ras (both plasmids are pBabe vectors) into primary mefs to make the mefs immortalized ? Would the E1A and Ras be integrated into the genome of the mefs ?

Thank you very much.

Go J Yoshida

Either Amaxa or Neon (both methods are electroporation) would be more effective than lipofectamine when it comes to MEF.

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