Can anyone recommend utilizing the plasmid extraction kit for extraction of the circular DNA genome of viruses associated with insect vectors?

Dear Colleagues, I am planning to detect and discover DNA viruses particularly those with a circular genome and transmitted by insect vectors. However, I am confused about selecting the best...

10 January 2021 9,534 4 View

Is there a certain way of making HEPES buffer w/ KCl?

I am trying to switch from NaCl to KCl in my buffer. As soon as I add 4M KCl solution to DI water and HEPES, it precipitates. Would heating the buffer alleviate the problem? Or should i add KCl to...

07 January 2021 1,104 1 View

What are some of the differences between a research article and a review article?

What are some of the differences between a research article and a review article? Thanks in advance for your replies!

29 December 2020 2,066 26 View

For experts in Gaussian 09 for windows?

Dear Friends: 64 bit Gaussian 09 software for windows is more expensive than 32-bit Gaussian 09. In our work, we deal with coordination compounds with about 50 atoms. So, can we use 32-bit...

27 December 2020 2,877 2 View

Suggestions for Journals related to Psychology and Computer Science?

I am looking for a journal, related to Psychology and Computer Science. IF < 2.0. If anyone has published some related work, or know any journal. that would be nice.

07 December 2020 1,277 3 View

FEM modeling of Joints/ Connection?

The attached Joint.png showed and example case of a joint (2 continuum members are connected). There are 3 characteristics of this, 1. There is a Gap: Cause small amount of rigid body rotation and...

07 December 2020 6,603 3 View

How to compute intermolecular interactions in mixture of solvent?

Hello Is it possible to compute intermolecular interactions in a mixture of solvent using DFT tools in Gaussian-09 or GAMESS US, DMOl3 or any other computational chemistry code? For example we...

05 December 2020 7,336 3 View

How can we set up Hydrothermal Liquefaction using solar/microgrid for extracting biofules?

Fo the conversion of biomass to biofuels, Hydrothermal Liquefaction is chosen.But the whole operation has to be from renewable energy source including the whole process most probably by solar and...

23 November 2020 9,432 2 View

Do you think that the emergence of CORONAVIRUS affects Investor trading behaviors? How? give examples?

any Thoughts on this question will help me Thanks

06 November 2020 7,654 4 View

Dynamics of cochlea membrane ?

04 November 2020 3,333 1 View

Anyone plz suggest relevant topic for research in image processing+Deep learning.?

I have selected brain tumor images ...but now found that already lots of research done n this topic.

03 March 2021 5,774 3 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

Do I need to strip the PVDF membrane before staining it with colloidal gold?

Dear Colleagues, After running Western blot on PVDF membrane and detection using ECL, I would like to stain my PVDF with colloidal gold to be able to allign the ECL image with total proteins on...

02 March 2021 7,829 3 View

Do you know any MR image dataset?

I would like to research on MR images (0.5T and 3T). Can you please suggest some websites that I can download dataset including both 0.5T and 3T MR images? Thank you.

02 March 2021 7,735 3 View

How to find the mode of classified ocean colour images?

Hello, I have classified 10 S3 OLCI images from the same area (althouth not the same size and/or quadrant) and I would like to do a final image using the mode of the pixel of these 10 images. I...

01 March 2021 9,874 3 View

How to threshold an image despite bright lighting, using ImageJ/Fiji?

Hi everyone, I am using Fiji to determine the percentage of the image covered by leaves, branches, or trunk, but i've encountered some difficulty when the sun hits parts of my image like the one...

27 February 2021 1,194 3 View

Trouble with Charting with Google Earth Engine (Technical)?

Hello. I have a problem with the charting in google earth engine. I created an image collection by combining 5 image collections of five parameters and using ee.Join.inner ( ) and .apply ( )...

26 February 2021 7,084 2 View

Will a 10xHis-tag prevent anti-MBP from binding to MBP?

I have a protein with a 10xHis-MBP tag on the N-terminus. I'm fairly certain I am expressing my protein, but I'm not getting a signal on my western blot. Is there a possibility that the 10xHis tag...

25 February 2021 8,404 3 View