Seonggyu Kwon

9 Questions 7 Answers 0 Followers

Questions related from Seonggyu Kwon

When drawing a phylogenetic tree with somatic mutations(SNPs), what is the role of tree simulation process?

Hello, I am working on drawing a phylogenetic tree with samples sharing some somatic mutations. The basic process of my work is similar to this article. Population dynamics of normal human blood...

15 October 2020 8,295 4 View

I would like to mix FBS and horse serum (1:1) when culturing cells, do you think it would change sequencing results?

Hello, I am doing fibroblast, stem cell culture for sequencing. (From primary culture to the end) Since FBS is expensive, there are some protocols that mix FBS and horse serum (1:1) I have done...

11 September 2020 8,345 3 View

Is it possible to culture primary cells from old (about 2-year-old) mouse?

I am trying to do primary culture from an old mouse (about 2-year-old) for some reason. Usually, I tried to culture myofibroblasts and dermal fibroblasts but failed continuously. The cells...

27 April 2020 7,631 0 View

How can I prevent primary cells from differentiating?

I am doing a primary culture, and I have some problems while culturing the cells. When I culture the cells from primary to passage 1 and 2... lots of cells differentiate and stop dividing before...

08 April 2020 8,283 7 View

Is there any way I can do spheroid culture with fibroblasts (not cancer cell)?

I have done spheroid culture with various cancer cell lines The spheroid was formed from single cells. The method was like the added picture (not curved bottom well, matrigel, etc) The question...

31 March 2020 9,984 3 View

How to separate mouse/human gastric/intestinal mucosa, submucosa, muscle, serosa?

I am trying to separate mouse/human gastric/intestinal mucosa, submucosa, muscle, serosa and want to do the primary culture for each tissue. Since the layers are thin, are there any ways to...

22 March 2020 3,166 3 View

Can I use this live/dead cell assay kit after I fixed the samples?

https://www.thermofisher.com/order/catalog/product/L3224 This is the live/dead cell assay kit I will use for my experiment. But I have to fix samples (cancer cells) before using this kit, maybe...

30 April 2019 3,656 3 View

How do I treat gold nanoparticles to cells?

I am treating gold nanoparticles to cells(cancer cells) and see the adhesion & effect of gold nanoparticles on cells. When I look at microscope, I find out that gold nanoparticles are...

11 April 2019 8,859 4 View

What is the difference between gold nanoparticle and nanocage when treated on cells?

I am working on treatment of gold nanoparticles (AuNPs) and gold nanocages on various cell lines (MSCs, Cancer cells, Cancer stem cells) I found out that the viability of the cells were decreased...

25 February 2019 3,898 3 View