Is there a stain that can be used to differentiate bacteria and fungi in a sample?

I am looking at the location of bacteria and fungi in the GI tract of isopods. I may do fluorescent in situ hybridisation but am looking for an easier stain. I was looking at acridine orange but I...

04 May 2018 8,809 3 View

Can someone give some advice on my in situ hybridisation protocol?

I have sections of tissue on slides and looking at the bacteria in the tissue, but haven't got any results. Can anyone give me some feedback on my protocol please: Day 1 1. Wash twice in 1X PBS...

02 March 2018 3,688 4 View

Fungal probes that will detect only fungi and not organism cells?

I am carrying out fluorescent in situ on the bacteria and fungi found in the GI tract of a eukaryotic organism. The bacteria is easy, EUB338 is a universal bacteria probe However the fungi appears...

10 November 2017 974 7 View

Why did I get RNA bands and what can I do about them?

I grew up bacterial isolates, then did DNA extraction using spin kit for soil, which I have used many times. This time I appear to have got more bands than just the genomic DNA, why would I have...

05 June 2017 2,341 1 View

What is AIMS microbiology journal like?

It has been suggested I put a paper in to publish in AIMS microbiology, however I have yet to find an impact factor for this journal. Has anyone got experience with it? How do I find out it's...

02 March 2017 1,434 17 View

How to interpret beta diversity, between groups and within groups?

I've had bioinformatics data from sequencing company but am struggling with the beta diversity analysis. I have 2 fish, one was fed a mixed diet (X) and the other fed a wood diet (W). I sent off...

08 September 2016 9,610 8 View

How to set original inoculum of Fungi?

I am working with both bacteria and fungi. I want to inoculate my sawdust with 10^9 cells. I can do this with bacteria by using thermal spectrophotometer 600 nm and adding 0.1% peptone water until...

02 March 2016 5,581 3 View

Purification of PCR products?

I have amplified my samples using ITS1F and ITS4 primers. The gel has a faint band under the main band, the PCR settings are right as they work on other samples. The faint band is the same as the...

01 February 2016 1,703 8 View

Any advice on why there is no band on gel for sample, but band for +ve control and poison PCR?

I have crushed up woodlice, serial diluted and spread plated and have growth of fungus on SDA+antibiotics. I have also done a DNA extraction and run PCR with fungal primers, bacterial primers and...

10 November 2015 8,071 7 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

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02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View