I want a bacterial suspension of 10^9 cells?

04 April 2016 1 9K Report

Ive only done this with Ecoli and the OD is ~1 at 600nm for 10^9 cells. But this is dependent on cell size so not all species are the same OD.

How do I find out the OD for 10^9 of other species?

Wolfgang Schechinger

Caroline, estimate your bacteria density with a Neubauer-Chamber (or hemocytometer) and correlate OD with cell count.

Badges
Science method

More Caroline Marden's questions See All

Is there a stain that can be used to differentiate bacteria and fungi in a sample?

I am looking at the location of bacteria and fungi in the GI tract of isopods. I may do fluorescent in situ hybridisation but am looking for an easier stain. I was looking at acridine orange but I...

04 May 2018 8,886 3 View

Can someone give some advice on my in situ hybridisation protocol?

I have sections of tissue on slides and looking at the bacteria in the tissue, but haven't got any results. Can anyone give me some feedback on my protocol please: Day 1 1. Wash twice in 1X PBS...

02 March 2018 3,760 4 View

Fungal probes that will detect only fungi and not organism cells?

I am carrying out fluorescent in situ on the bacteria and fungi found in the GI tract of a eukaryotic organism. The bacteria is easy, EUB338 is a universal bacteria probe However the fungi appears...

10 November 2017 1,061 7 View

Why did I get RNA bands and what can I do about them?

I grew up bacterial isolates, then did DNA extraction using spin kit for soil, which I have used many times. This time I appear to have got more bands than just the genomic DNA, why would I have...

05 June 2017 2,437 1 View

What is AIMS microbiology journal like?

It has been suggested I put a paper in to publish in AIMS microbiology, however I have yet to find an impact factor for this journal. Has anyone got experience with it? How do I find out it's...

02 March 2017 1,520 17 View

How to interpret beta diversity, between groups and within groups?

I've had bioinformatics data from sequencing company but am struggling with the beta diversity analysis. I have 2 fish, one was fed a mixed diet (X) and the other fed a wood diet (W). I sent off...

08 September 2016 9,758 8 View

How to set original inoculum of Fungi?

I am working with both bacteria and fungi. I want to inoculate my sawdust with 10^9 cells. I can do this with bacteria by using thermal spectrophotometer 600 nm and adding 0.1% peptone water until...

02 March 2016 5,699 3 View

Purification of PCR products?

I have amplified my samples using ITS1F and ITS4 primers. The gel has a faint band under the main band, the PCR settings are right as they work on other samples. The faint band is the same as the...

01 February 2016 1,775 8 View

Any advice on why there is no band on gel for sample, but band for +ve control and poison PCR?

I have crushed up woodlice, serial diluted and spread plated and have growth of fungus on SDA+antibiotics. I have also done a DNA extraction and run PCR with fungal primers, bacterial primers and...

10 November 2015 8,153 7 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

How to use Density Functional Theory to calculate carrier mobilities of solid system?

Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...

04 August 2024 8,894 1 View