Is there a stain that can be used to differentiate bacteria and fungi in a sample?

More Caroline Marden's questions See All

Can someone give some advice on my in situ hybridisation protocol?

I have sections of tissue on slides and looking at the bacteria in the tissue, but haven't got any results. Can anyone give me some feedback on my protocol please: Day 1 1. Wash twice in 1X PBS...

02 March 2018 3,688 4 View

Fungal probes that will detect only fungi and not organism cells?

I am carrying out fluorescent in situ on the bacteria and fungi found in the GI tract of a eukaryotic organism. The bacteria is easy, EUB338 is a universal bacteria probe However the fungi appears...

10 November 2017 974 7 View

Why did I get RNA bands and what can I do about them?

I grew up bacterial isolates, then did DNA extraction using spin kit for soil, which I have used many times. This time I appear to have got more bands than just the genomic DNA, why would I have...

05 June 2017 2,341 1 View

What is AIMS microbiology journal like?

It has been suggested I put a paper in to publish in AIMS microbiology, however I have yet to find an impact factor for this journal. Has anyone got experience with it? How do I find out it's...

02 March 2017 1,434 17 View

How to interpret beta diversity, between groups and within groups?

I've had bioinformatics data from sequencing company but am struggling with the beta diversity analysis. I have 2 fish, one was fed a mixed diet (X) and the other fed a wood diet (W). I sent off...

08 September 2016 9,610 8 View

I want a bacterial suspension of 10^9 cells?

Ive only done this with Ecoli and the OD is ~1 at 600nm for 10^9 cells. But this is dependent on cell size so not all species are the same OD. How do I find out the OD for 10^9 of other species?

03 April 2016 9,176 1 View

How to set original inoculum of Fungi?

I am working with both bacteria and fungi. I want to inoculate my sawdust with 10^9 cells. I can do this with bacteria by using thermal spectrophotometer 600 nm and adding 0.1% peptone water until...

02 March 2016 5,581 3 View

Purification of PCR products?

I have amplified my samples using ITS1F and ITS4 primers. The gel has a faint band under the main band, the PCR settings are right as they work on other samples. The faint band is the same as the...

01 February 2016 1,703 8 View

Any advice on why there is no band on gel for sample, but band for +ve control and poison PCR?

I have crushed up woodlice, serial diluted and spread plated and have growth of fungus on SDA+antibiotics. I have also done a DNA extraction and run PCR with fungal primers, bacterial primers and...

10 November 2015 8,071 7 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

How to remove the residual PDMS on the silicon mold?

Incompletely cross-linked pdms are difficult to remove.

01 March 2021 798 3 View

How to highlight novelty in your research paper?

I have come up with a method and had written a paper on it. Paper got rejected by a journal stating that there is no theoretical novelty. I am kind of confused on why the novelty that I am able to...

28 February 2021 2,393 8 View

How to define tie constraint between rigid body and another part in dynamic explicit model of Abaqus?

Hi dear researchers I connected a rigid body into the part of my structure by tie constraint and then I added displacement to rigid body, in static general step every thing is fine and these two...

28 February 2021 3,442 2 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.

27 February 2021 9,356 3 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View