Caroline Marden

10 Questions 15 Answers 0 Followers

Questions related from Caroline Marden

Is there a stain that can be used to differentiate bacteria and fungi in a sample?

I am looking at the location of bacteria and fungi in the GI tract of isopods. I may do fluorescent in situ hybridisation but am looking for an easier stain. I was looking at acridine orange but I...

05 May 2018 8,872 3 View

Can someone give some advice on my in situ hybridisation protocol?

I have sections of tissue on slides and looking at the bacteria in the tissue, but haven't got any results. Can anyone give me some feedback on my protocol please: Day 1 1. Wash twice in 1X PBS...

03 March 2018 3,748 4 View

Fungal probes that will detect only fungi and not organism cells?

I am carrying out fluorescent in situ on the bacteria and fungi found in the GI tract of a eukaryotic organism. The bacteria is easy, EUB338 is a universal bacteria probe However the fungi appears...

11 November 2017 1,040 7 View

Why did I get RNA bands and what can I do about them?

I grew up bacterial isolates, then did DNA extraction using spin kit for soil, which I have used many times. This time I appear to have got more bands than just the genomic DNA, why would I have...

06 June 2017 2,407 1 View

What is AIMS microbiology journal like?

It has been suggested I put a paper in to publish in AIMS microbiology, however I have yet to find an impact factor for this journal. Has anyone got experience with it? How do I find out it's...

03 March 2017 1,503 17 View

How to interpret beta diversity, between groups and within groups?

I've had bioinformatics data from sequencing company but am struggling with the beta diversity analysis. I have 2 fish, one was fed a mixed diet (X) and the other fed a wood diet (W). I sent off...

09 September 2016 9,742 8 View

I want a bacterial suspension of 10^9 cells?

Ive only done this with Ecoli and the OD is ~1 at 600nm for 10^9 cells. But this is dependent on cell size so not all species are the same OD. How do I find out the OD for 10^9 of other species?

04 April 2016 9,234 1 View

How to set original inoculum of Fungi?

I am working with both bacteria and fungi. I want to inoculate my sawdust with 10^9 cells. I can do this with bacteria by using thermal spectrophotometer 600 nm and adding 0.1% peptone water until...

03 March 2016 5,674 3 View

Purification of PCR products?

I have amplified my samples using ITS1F and ITS4 primers. The gel has a faint band under the main band, the PCR settings are right as they work on other samples. The faint band is the same as the...

02 February 2016 1,764 8 View

Any advice on why there is no band on gel for sample, but band for +ve control and poison PCR?

I have crushed up woodlice, serial diluted and spread plated and have growth of fungus on SDA+antibiotics. I have also done a DNA extraction and run PCR with fungal primers, bacterial primers and...

11 November 2015 8,133 7 View