Why did I get RNA bands and what can I do about them?

06 June 2017 1 2K Report

I grew up bacterial isolates, then did DNA extraction using spin kit for soil, which I have used many times.

This time I appear to have got more bands than just the genomic DNA, why would I have got this and what can I do?

I think there was a lot of starting bacteria scraped off the plates, the nano drop showed an error message so I couldn't get readings but I suspect they are high.

Thanks

Mohd Shahnawaz

I agree with Eric Marques, you should contact the kit manufacturer. Further before contacting, kindly check expiry date of RNase. I too used many kits such as Qiagen, Banglore Genei and Himedia etc and did not got promising results. So, based on experience in bacterial DNA isolation, I recommend basic CTAB method for the best quantity and quality.

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