Biorad precast gels Tris-Tricine

Loading buffer with Orange G

Fixation: 1 h 5 % glutaraldehyde

Rinse: 2 x 20', overnight, 1 x 20 ' A. bi dest

Sensitization: 0,2 g/L ASA 1'

Rinse: 3 x 20 ''

silver: silver nitrate 0,2 %, 37 % HCHO 0,7 mL/L 45'

rinse: water 10 ''

Development: 3% potassium carbonate, 37 % HCHO 0,25 mL/L , STS 10 mg/L 3'

STOP: 5% Acetic acid