Differences of DNA replication and repair between prokaryotes and eukaryotes?

I require differences of DNA replication and repair between prokaryotes and eukaryotes mutually exclusive in both

09 October 2018 198 2 View

In what condition Caspase 3Cleaved would be positive even before Caspase 8?

07 August 2016 7,962 3 View

How to use ImageJ software?

I need to quantify the stained picture using ImageJ please let me know step wise how to make use of it. How can i get the area stained ?

03 April 2016 8,530 3 View

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31 December 2015 373 3 View

Can Bio Rad Gel doc be used for visualization of western blot?

31 December 2015 4,356 4 View

What consideration to be taken for choosing endonucleases?

You are given the following assignment by a biotechnology firm. transfer gene A from a mouse chromosome to plasmid pBR322 of Ecoli. What would be your first consideration in choosing in one or...

10 November 2015 2,275 0 View

How to cultivate human PBMC in culture flask?

Can some body provide protocol to cultivate human PBMC in cell culture ?

06 July 2015 7,093 4 View

Can any body suggest how to solubilize bacterial protein extracted by acetone precipitation?

03 April 2015 4,481 4 View

Can anybody provide a protocol for protein extraction of staph aureus bacteria?

Can anybody provide me with the best protocol to be used for protein extraction form staph aureus?

01 February 2015 7,465 3 View

Can I store blotting paper after treating with ECL pierce, in case I want to develop it later on with X ray film?

How long can I wait to develop an x ray film after my blotting paper is treated with ECL pierce (Chemiluminescence).

31 December 2014 9,152 4 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

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02 March 2021 3,060 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View