I am stimulating a J774 cell line and need to check interleukin's expression please provide me a good protocol. How can I get good extract to evaluate interleukin's levels in cell line?
I require differences of DNA replication and repair between prokaryotes and eukaryotes mutually exclusive in both
09 October 2018 341 2 View
I checked the apoptotic effect of a natural molecule on cell line. In my western result I do get over expressed PARP Clvd and caspase 3 positive at 24 hours after treatment. When i checked level...
07 August 2016 8,084 3 View
I need to quantify the stained picture using ImageJ please let me know step wise how to make use of it. How can i get the area stained ?
03 April 2016 8,715 3 View
we do have Gel doc which can be used to see Agarose gel stained with EDBR for DNA i want to know same set can we use for western blot protein visualization without any other accessories? ( using...
31 December 2015 4,498 4 View
we need Image Lab™ Software #1709690 for bio rad to visualize chemiluminescence, the original software actually got corrupted and getting from company is quite costly any other way to get it?
31 December 2015 505 3 View
You are given the following assignment by a biotechnology firm. transfer gene A from a mouse chromosome to plasmid pBR322 of Ecoli. What would be your first consideration in choosing in one or...
10 November 2015 2,406 0 View
Can some body provide protocol to cultivate human PBMC in cell culture ?
06 July 2015 7,301 4 View
I am working to extract the whole protein from staphylococcus aureus. the steps include treating cell pallets with lysostaphine followed by water bath sonication. The collected supernatant is...
03 April 2015 4,608 4 View
Can anybody provide me with the best protocol to be used for protein extraction form staph aureus?
01 February 2015 7,605 3 View
How long can I wait to develop an x ray film after my blotting paper is treated with ECL pierce (Chemiluminescence).
31 December 2014 9,304 4 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you
08 August 2024 4,733 2 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View