I have synthesized silver nanoparticles by using plant leaf extract, but no nanoparticles are found after incubation or settling?

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What is considered a biological replicate when you are doing an ELISA plate measurement with different cell types?

If cells obtained from different passages, with different plating but harvested on the same day are used in a single plate to take an ELISA measurement (with the given standards and treated...

17 June 2024 6,705 1 View

Which phosphor antibodies to choose to study the hypo and hyperphosphorylation state of the SRSF1 protein?

I am trying to understand the different phosphorylation states of SRSF1 protein in the cytoplasm vs nucleus and looking for specific antibodies for the phosphor state. As SR proteins include...

11 February 2024 7,242 3 View

Why does sauce(oyster sauce) because water?

the company that I'm working in produces sauce and we notice there is watery issue in our oyster sauce after bottling although the sauce is within its validated shelf life.

09 May 2023 5,977 1 View

Is it necessary to change media after transfecting cells with Lipofectamine3000?

I transfected HCt116 cells with GFP using Lipo3000. I didn't change media after transfection as the manufacturer guidelines say not necessary. However, I noticed a change in the cell morphology...

28 April 2023 6,071 6 View

Tafel analysis clarification and its reference?

I have done potentiodynamic polarization (Tafel) studies for a few surface modified samples. The intercepts and slopes were done exactly by the origin software. Its results show a gradual...

06 April 2023 1,226 1 View

Is Native RNA immunoprecipitation performed without cross linking?

I am trying to study the RNA-protein interactome of a specific protein using IP studies. I need to use native IP for my purpose and wanted to know if native RIP is without cross-linking study and...

01 February 2023 915 0 View

Is loading equal volume of protein in SDS PAGE correct?

I am comparing the protein expression levels in subcellular fractionations (Cytoplasm and nuclear). If I load an equal volume of both extracts (15 ul each to individual wells), is this still valid...

26 January 2023 6,257 3 View

How to use CRISPOR software for design guide RNA?

I want to know tutorial help to design and how to choose suitable g for knok out

29 July 2022 4,099 2 View

Which region of the gene should I target for a CRISPR mediated Knockout of Target gene?

I want to design g RNA for knock out pathway gene to enhance secondary metabolites

29 July 2022 8,077 0 View

What is the right media for HCT116?

Is DMEM or DMEM/F-12K the right media for culturing HCT116 cells apart from McCoys media? Did anyone use the gibco one? I see a lot of dead cells when using DMEM. It would be helpful if someone...

20 June 2022 1,059 3 View

Hello researchers Is this a random laser or just fluorescence?

I am using Rhodamine6G as gain medium and silver nanoparticles as scatterers on a microscope slide and laser input 532 nm comes from above.

09 August 2024 9,894 2 View

How to prepare the nanoparticle treated fungal sample for Environmental SEM analysis?

A fungal strain was treated with nanoparticles. We want to do an environmental SEM analysis. So could anyone share your views on preparing the sample? Thank you.

07 August 2024 5,307 1 View

Why results of ROS flurescence are negative as there was no bacteria within?

Hello. I am working on ROS production of two systems: system A is cerium oxide and hydrogen peroxide, system B is cerium oxide nanoparticle, hydrogen peroxide and potassium bromide. I did some...

04 August 2024 5,974 3 View

Binder for ZnO nanoparticles in graphite paste electrode?

I have been using paraffin, but the deposited ZnO still detach from electrode. What is the best binder to modify graphite paste electrode with ZnO nanoparticles?

03 August 2024 4,624 3 View

Can i quantify a nanoparticle encapsulated in cell culture medium by hplc?

Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...

03 August 2024 2,730 1 View

Some new emerging problems on application of RL for scheduling in IoT networks?

I have seen plenty of existing works on applied Reinforcement Learning (RL) policies for optimized scheduling in IoT networks including Q-learning, DQNs, and the newer ones including PPO for...

01 August 2024 8,754 2 View

What is the best practice for resuspending cell pellets during competent cell preparation (both electro and comp)?

I'm fairly new to making homemade comp cells and have done so with some mild success. What is generally an acceptable way of resuspending comp cells during wash steps? I've been told to swirl the...

31 July 2024 8,309 0 View

Mossbauer spectroscopy Co0.6Mn0.4Fe2O4 MNPs?

Following curve related to Co0.6Mn0.4Fe2O4 Magnetic nanoparticles with extra phase a-Fe2O3. I will appreciate to help me to interpretation this spectroscopy, especially the blue line.

31 July 2024 9,140 2 View

Hi there, someone has the SeinFit software for windows because I cannot download it?

DOS version.

29 July 2024 6,064 1 View

There is possible way to calculate experimental DOS from the UPS spectrum of the material. How to calculate the DOS from UPS?

Here, I have attached the UPS graph. I'm trying to calculate the DOS/DOVS from the UPS.

29 July 2024 4,971 1 View