Sandesh S Mandavkar

12 Questions 14 Answers 0 Followers

Questions related from Sandesh S Mandavkar

FTIR transmittance is more than 100%. I want to know why and how to solve it?

Hi, I am working on marine natural products and currently characterizing antimicrobial compounds. I dissolved my sample in DMSO and was submitted to FTIR. In result, FTIR transmittance spectrum is...

25 January 2018 7,270 26 View

How to calculate value of [S] for amylase?

While doing enzyme kinetics, we need to have molar concentration of substrate for carrying out Michalis Menten kinetics. But in the case of starch, since it is a polysaccharide, molecular weight...

25 April 2016 9,068 4 View

What should be the concentration of sodium carbonate solution used in phenolphthalein assay?

I am about to check cyclodextrin glycosyl transferase detection. In detection protocol, its been mentioned that 4 mL of 0.04 mM phenophthalein solution in sodium carbonate. The concentration of...

20 February 2016 611 2 View

How do you get rid of an oil globule-like substance forming during organic solvent extraction?

Hi, I have started with organic solvent extraction of spent medium or cell free supernatant with butanol, but an oil globule-like substance is being formed in organic layer. I want to know how to...

11 March 2015 1,497 4 View

Which layer should be selected in organic solvent extraction of cell free supernatent ?

Hi, I have started with organic solvent extraction of cell free supernatent and I am getting two layers. Upper layer and bottom layer. The organic solvents used are Chloroform, Ethyl acetate,...

11 March 2015 7,637 0 View

What is the factor responsible for the black color of the enriched zobells marine broth 2216?

HI, I am trying to isolate marine bacteria using zobells marine broth 2216. Before inoculation the color of zobells marine broth 2216 was same as nutrient broth but on incubation after 3-5 days it...

23 December 2014 3,797 4 View

What is the significance of glycerol in sea water agar?

Hi, I am trying to isolate marine bacteria from marine sediments. Since zobell marine broth is not available, i have started with sea water agar. In one of the sea water agar, glycerol have been...

11 August 2014 2,129 2 View

What is the ideal temperature for organic solvent extraction as per the petroleum ether is concerned?

I am about to do organic solvent extraction but I am confused which one to use. The one with a temperature range of 40-60, 60-80 or 80-100.

11 April 2014 7,587 8 View

What is the bioactivity guided fractionation of natural products and does it apply to bacterial products?

Hi guys, I am planing to work on a marine bioactive compound. I came across a bioactivity guided protocol. Now I am confused about the fraction to be used for bioactivity. Which fraction...

19 March 2014 527 2 View

What is the ideal glycerol concentration for glycerol stock of bacterial culture?

I carried out glycerol stock preparation of bacterial culture using 20% glycerol. I left it for night in a refrigerator. When I checked net morning, the suspension inside the vial was found to be...

13 March 2014 588 35 View

Can anybody refer me to a group working on anti-tubercular activity?

I am interested in anti-tubercular and want to know about a group working on it.

06 March 2014 9,847 2 View

Can anyone please tell me about the site from which sponge samples can be collected from Mumbai coastal region?

I am working on the symbiotic association of marine bacteria and sponges.

13 October 2013 3,534 1 View