Sandesh S Mandavkar

9 Questions 9 Answers 0 Followers

Questions related from Sandesh S Mandavkar

FTIR transmittance is more than 100%. I want to know why and how to solve it?

Hi, I am working on marine natural products and currently characterizing antimicrobial compounds. I dissolved my sample in DMSO and was submitted to FTIR. In result, FTIR transmittance spectrum is...

25 January 2018 7,231 26 View

What should be the concentration of sodium carbonate solution used in phenolphthalein assay?

I am about to check cyclodextrin glycosyl transferase detection. In detection protocol, its been mentioned that 4 mL of 0.04 mM phenophthalein solution in sodium carbonate. The concentration of...

20 February 2016 576 2 View

How do you get rid of an oil globule-like substance forming during organic solvent extraction?

Hi, I have started with organic solvent extraction of spent medium or cell free supernatant with butanol, but an oil globule-like substance is being formed in organic layer. I want to know how to...

11 March 2015 1,453 4 View

Which layer should be selected in organic solvent extraction of cell free supernatent ?

Hi, I have started with organic solvent extraction of cell free supernatent and I am getting two layers. Upper layer and bottom layer. The organic solvents used are Chloroform, Ethyl acetate,...

11 March 2015 7,608 0 View

What is the factor responsible for the black color of the enriched zobells marine broth 2216?

HI, I am trying to isolate marine bacteria using zobells marine broth 2216. Before inoculation the color of zobells marine broth 2216 was same as nutrient broth but on incubation after 3-5 days it...

23 December 2014 3,756 4 View

What is the ideal temperature for organic solvent extraction as per the petroleum ether is concerned?

I am about to do organic solvent extraction but I am confused which one to use. The one with a temperature range of 40-60, 60-80 or 80-100.

11 April 2014 7,533 8 View

What is the bioactivity guided fractionation of natural products and does it apply to bacterial products?

Hi guys, I am planing to work on a marine bioactive compound. I came across a bioactivity guided protocol. Now I am confused about the fraction to be used for bioactivity. Which fraction...

19 March 2014 484 2 View

What is the ideal glycerol concentration for glycerol stock of bacterial culture?

I carried out glycerol stock preparation of bacterial culture using 20% glycerol. I left it for night in a refrigerator. When I checked net morning, the suspension inside the vial was found to be...

13 March 2014 481 35 View

Can anybody refer me to a group working on anti-tubercular activity?

I am interested in anti-tubercular and want to know about a group working on it.

06 March 2014 9,818 2 View