I have done its GCMS. But i am interested in isolation of active compounds. Whether i should go for TLC, HPLC. plz comment
Dear Researcher,
Fractionate with n-hexane, chloroform, ethyl acetate and n-butanol and screen the fractions for activities of your interest.
I think a thorough literature review on the said drug will give you a hint of the kind of activity the drug has.Estimation of the compounds can be carried out after GCMS.It really depends on what activity you are interested in/are expecting.
Hi Shazia,
There are multiple bioactive compounds that could be present in your plant of interest: phytochemicals, polysaccharides, proteins, fatty acids etc. But since you have extracted with ethanol (a polar solvent), my guess is that you are interested in bioactive phytochemicals as these are soluble in polar solvents. Preliminary phytochemical screening as reported by Trease and Evans (2002), Also, HPLC, TLC and FTIR could be used. I have attached a review which explain in detail, the pros and cons of the methods
Regards,
Chigozie
Thanks Chigozie
I was searching this paper but it was not opening here. It is really helpful.
Actually i wanted to have terpenoids, after studying the literature , i selected ethanol as a solvent for plant extraction. Now, after extraction, i did GCMS, that identified nonpolar terpenoids and some other compounds. The plant name is Prosopis Juliflora. So, i am a little bit surprised as ethanol extracts polar compounds then from where these nonpolar terpenoids are coming? Plz comment
Regards
Dear Shazia,
Polarity concepts are not absolute in almost cases. Polar solvent can solvate apolar compounds and apolar compounds can solvate polar compounds. But, if you use polar compounds you expect solubilize polar compounds in higher proportions, chemical theory said, additionally you are going to obtain apolar compounds solvated (very low proportion normally).
Regards,
Hi Shazia,
In addition to Elkin's comment, Solvents differ in polarity, for example water, n hexane, ethanol and diethyl ether differ in polarity. Hence, if you could fractionate the ethanol extract using any or all of these solvents and compare the phytochemicals they extract. My guess is the range of compounds you are looking for will be different and your compound of interest may be in one of them.
Regards,
Chigozie
How to fractionate ethanol extract with n-hexane, water and diethyl ether?
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