I have conducted a Moderation analysis with no interaction effect. Should I change analysis to Multiple Regression?

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Has anyone encountered any problems with abm good microRNA primers for quantification of mature miRNAs?

I've been trying to quantitate mature microRNAs in 293T cells with and without 'X'. 2ug of total RNA (isolated by Trizol method) is converted to cDNA using All-in one microRNA cDNA synthesis kit...

17 February 2021 8,986 2 View

Are the molecular biology reagents of HiMedia ( kits,pcr and electrophoresis products) as good as products of NEB, Sigma and Invitrogen?

HiMedia seems to be cheaper than the rest.Anybody who has used it for molecular biology experiments, how is the quality?

26 September 2019 7,010 3 View

Annealing temperature in RT PCR - Positive control differes from target gene?

The primer annealing temperature , after the gradient PCR analysis, for the target gene differs from the optimal annealing temperature in housekeeping gene (positive control). What to do ? The...

02 July 2019 810 3 View

What statistical test can i use on my data?

I am researching the relationship between animal biodiversity and physics-chemical properties of a clean and polluted burrow dyke. I have the species abundance for both burrow dykes, as well as...

26 June 2019 569 2 View

Segregation coefficient of self-compacting concrete and small superplasticizer overlaps?

What do you think how important, for segregation coefficient of SCC, could be very small superplasticizer overlaps during the process of making SCC? I made some observations in my work: Some...

04 May 2019 6,951 4 View

I was only able to generate forward sequences for some of my matK, rbcL and ITS1 genes and will they still be useful for my phylogenetic analysis?

I sent some plant DNA for DNA sequencing and apparently, though sequences were sent back, some pairs don't create a consensus sequence. The sequencing company tried to re-sequence, bu to no...

26 April 2019 2,664 4 View

What policies may bring about sustainable reform and development?

Social disparities rise amid volatile economic and political times today. What may be constructive efforts in policy form to start resolving issues that cause such state of affairs?

16 March 2019 1,086 4 View

How can I improved the power (accuracy) of my Deep Learning Model?

Hey everyone, me and my colleagues are programming a Deep Learning Model with tensorflow. Our goal is it to predict chemical toxicity. Our input are chemical drugs in SMILE-Strucuture. So, how...

07 June 2018 7,920 3 View

When collecting qualitative data, what systems do people use to keep track of the different data collected?

I would like to know if people have concrete suggestions about or know of bibliography or on line resources that describe how to keep track of the different data collected. For example, if...

02 March 2018 5,892 9 View

What are uses of waste in geotechnical engineering?

waste use in improving soil engineering specification such as disaster waste, cement waste and others

02 August 2017 8,663 16 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Logistical regression using SPSS for a dichotomous dependent variable, with an independent variable of 1, 2, 5 and 10 seconds?

I have an experiment with 28 participants The independent variable is the times 1, 2, 5, and 10 used as the cross hairs during a task These 4 times were tested using 4 different blocks, each...

03 March 2021 9,692 2 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

What degree of missing data in a particular case/participant results in Expectation-Maximisation no longer working?

I'm dealing with a mediation model and am using the PROCESS module in SPSS. Due to SPSS and PROCESS being limited in the imputation methods - being unable to handle multiple imputation - the other...

02 March 2021 4,362 1 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View