We are now conducting Inter-laboratory test program. Would like to evaluate the Accuracy and Precision of each lab. For accuracy, we will use z-score.
May I know how to calculate the Precision for repeatability and reproducibility?
A good starting point maybe the following article: Uncertainty evaluations from small datasets by Sara Stoudt, Adam Pintar and Antonio Possolo, in Metrologia, Volume 58, Number 1, DOI 10.1088/1681-7575/abd372
I am trouble shooting a malachite green ATPase assay using a kit from Sigma (MAK307) for a cytosolic protein that converts ATP --> ADP + Pi and am wondering what cheap + commercially available...
30 March 2024 9,659 1 View
In the Journal "The transition from gel separatory serum tubes to lithium heparin gel tubes in the clinical laboratory" the researchers found discrepancies in regard to the effects of plasma...
25 February 2024 9,949 2 View
I have to treat the mice with a drug that is dissolved in DMSO, but to decrease the percentage of DMSO I want to add PEG400 and cyclodextrin. I would like to know the maximum recommended...
17 January 2024 8,543 1 View
Using EGFP fusion protein positioning, it was found that there are linear luminophores in the hyphae of Fusarium. What subcellular structure is this?
12 January 2024 4,557 0 View
The optimal dosing regimen for antibiotics used in combination therapy against drug-resistant bacteria should be determined through careful consideration of these factors, along with ongoing...
23 February 2023 6,965 2 View
Hi everyone, I'm troubleshooting using frozen ascites for different techniques i.e. cell culture and immunoblotting. However, when the ascites specimens were collected they were put at -80 C in...
07 November 2022 6,487 0 View
Hi all, It's my first time culturing NK cells, not only mine but also the first time it is being done in my lab so I am kind of on my own. Does anyone have a step-by-step protocol they could...
23 March 2022 6,008 1 View
I'm using the Miltenyi Biotec NK MACS medium however the protocol says to use 5% AB Serum, is it possible to substitute the HS for HSA? IL-2 and IL-15 will be added to the media to help with NK...
22 March 2022 6,194 3 View
I have been using Prolong Gold with DAPI for my IF staining. However the last few times no DAPI signal has been showing up. Only problems I can think of is that I let the gel curate at 4C and...
21 July 2021 8,349 1 View
I am currently working on a project on local nonlinear effects. Comfortably solved the problem using harmonic balance method. Then I move on to write my own code based on Newton Raphson method....
14 June 2021 5,982 4 View
Program: g_mmpbsa, version 2024.1 Source file: extrn_apbs.cxx (line 152) Fatal error: Failed to execute command: $APBS pybYcUWA.in --output-file=pybYcUW.out
07 August 2024 6,066 0 View
I want a model that is balanced with accuracy or speed, faster rcnn has high accuracy while yolo have fast speed. i am thinking to combine them to get a hybrid model to achieve both speed and accuracy
02 August 2024 3,104 0 View
Are students in the MBA program in Fisheries Enterprises Management eligible to work in the aquaculture industry abroad? Could you explain the foreign countries interested in recruiting...
28 July 2024 8,378 2 View
How can we calculate the percentage of configuration interaction (CI) in the UV output data of the Gaussian program? for example: Excited State 17: Singlet-A 5.1359 eV 241.41 nm...
28 July 2024 9,165 2 View
I need to install this program
25 July 2024 4,756 0 View
Do You Believe VP Harris Would Show Empathy to Tech Companies in Her Administration as POTUS? I ask in light of the fact that, the ACP was not re-funded this round. So many and businesses need...
24 July 2024 4,732 3 View
Dear all, I gave 116 respondents 18 translated sentences and asked them to indicate their levels of acceptance of these translations on a five-point scale. Some translations result from strategies...
24 July 2024 8,245 5 View
Hello, colleagues! There is commenting open for new upcoming edition of USP 1033. Validation target acceptance criteria is now different from what it used to be and it doesn't include Cpm....
23 July 2024 7,292 3 View
Dear All: I have to quantify the fluorescence intensity of cells that are fluorescent to two different markers, so i'm interested in finding out if this is possible. Previously I have...
22 July 2024 8,770 1 View
Using DFT/B3LYP/6-311++G
17 July 2024 7,720 1 View