Does anyone know if the Quantstudio 3 qPCR machine requires a passive reference when Using SYBR? I ran a qPCR and got the plot in the attached file.?

I know that my target is in my sample as I extracted it from cell culture myself. These plots show some amplification. The first one seems O.K for the first 36 cycles but then collapses at the...

14 February 2021 2,809 2 View

Ferret PBMC Fc block?

Hi all, I'm going to run Flow Cytometery on ferret PBMCs but can't find an Fc block for ferret PBMCs. Does that even exist? Do I need ferret-specific Fc blocking antibodies or can I use:...

18 January 2021 4,504 5 View

Platelet Counting on Flow Cytometry?

Hi, Does anyone have a method for counting platelets on flow cytometry (cytometer=Cytoflex) without the need of using TRUcount beads or reference beads? Is it possible or do I need to purchase...

23 September 2020 3,509 1 View

What is the best bibliographic output style for preparing a CV?

I am looking for an output style in Endnote to list publications in one's CV. These would have to be be listed in reverese chronological order. Recommendations and opinions welcome.

23 March 2020 1,228 4 View

Anyone using stereo trail cameras (camera traps) linked to gather morphometric data from cryptic species?

Looking to connect with any research, papers or groups using remotely triggered stereo cameras to create 3 dimensional pictures of wildlife and gather data on cryptic species. Wanting to set up a...

01 February 2020 940 3 View

Finding EA(energy absorption)?

Hello every one, I am new to abaqus. How do I find energy indicators like, EA(energy absorption), Mean Crush Force (MCF) or Crush Force Efficiency (CFE) in a result section for a buckling process...

05 May 2019 5,193 3 View

I was only able to generate forward sequences for some of my matK, rbcL and ITS1 genes and will they still be useful for my phylogenetic analysis?

I sent some plant DNA for DNA sequencing and apparently, though sequences were sent back, some pairs don't create a consensus sequence. The sequencing company tried to re-sequence, bu to no...

26 April 2019 2,664 4 View

What am I doing wrong - extracting a sapogenin for NMR?

I use hydrochloric acid to release a steroidal sapogenin from its saponin, then neutralise it with sodium bicarbonate. I am confident the hydrolysis works because the mixture no longer produces a...

03 June 2018 3,814 3 View

Wrist Laxity carpal kinematics: Anyone know where I can find this conference abstract?

My library cannot locate: Effects of wrist laxity on three-dimensional carpal kinematics. J Orthop Res Conf. 2017,35(Supplement 1). by Stoop, Best, Pichora, Kamal, Rainbow Any assistance in...

24 April 2018 2,543 3 View

When collecting qualitative data, what systems do people use to keep track of the different data collected?

I would like to know if people have concrete suggestions about or know of bibliography or on line resources that describe how to keep track of the different data collected. For example, if...

02 March 2018 5,892 9 View

Research on Supply Chain Management in Indian context?

Hi, I am planning to apply for the PhD degree in the Supply Chain Mgt. with specific area of "Cold Storage warehouses" during Pandemics and wars. Where lock downs and shut downs are frequent....

02 March 2021 285 2 View

Can you share me how teacher education is managed in your respective national educational system?

I feel that the practice in teacher education in my country is below the expected performance level due to very poor management system. Hope I will learn something from your experiences.

02 March 2021 1,516 4 View

How does one access the Cranet survey data on HRM practices?

Hello, The Cranet website does not provide any lead to the data obtained from their multinational survey on HRM practices. Any help on accessing the data would be most appreciated. Thank...

01 March 2021 9,536 3 View

Is there any research regarding how training and development is going to change in the era of Fourth Industrial Revolution?

As the organizations getting prepared for the fourth industrial revolution, to enhance the skills of their workforce what type of changes are necessary and going to occur in the training and...

01 March 2021 3,199 3 View

Which is suitable for use with Python? MySQL or SQL Server?

Which is suitable for use with Python? MySQL or SQL Server? What is your suggestion?

01 March 2021 3,422 3 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

What role should journals play in leading academic fields?

A recent paper by Yeung and Thomas (2021) on journals in event management opens up an interesting debate on the role of journals as academic fora. They demonstrate that event management publishing...

28 February 2021 3,494 6 View

How to add missing citation?

Dear colleagues, I have found that many authors have a concern regarding their missing citation in RG because often citation in RG is lower than google scholars. I also missed some citation...

26 February 2021 928 6 View

Where i will get the Desmodium and Napier grass seeds?

26 February 2021 975 4 View

How vulnerable is water and wastewater infrastructure to cyber terrorism in an age of IoT?

Im looking for any papers, reports,articles, links etc on information on How vulnerable is water and wastewater infrastructure to cyber terrorism in an age of IoT.

24 February 2021 2,750 4 View