Mtt assay
Dear Zohreh Zarei Ghane Use trypan blue exclusion assay with MTT assay. Then you can know if there is any over/under-estimation of your results.
CT26 cell line . i would like to measure IC50.
Hello, I have a script changing the distances of two bonded atoms and I calculated the total energy (or potenial energy, kinetic energy is zero) with energy minimisation tool of lammps (ReaxFF...
27 March 2024 4,468 2 View
need the latest articles on the powerful use of body language among politicians for policy conception and how politicians leverage body language to influence public opinion and gain support.
10 March 2024 5,036 2 View
hi every one it is my first time I want to send cells for flowcytometry. I want to be sure is it okay to seed cells in 6 well plate for both apaptotic assay and cell cycle assay just in one...
26 January 2024 4,836 0 View
"Please help me answer this question."
25 January 2024 2,768 4 View
I need to wash glass ampoules to remove organic residues and other chemical contaminant. Which kind of acid and which concentration of it is needed?
06 January 2024 4,266 3 View
transfection, lipofectamine 2000
14 November 2023 2,379 4 View
Hi I have problem importing thesaurus file to vosviewer. the error is ".... is not valid column header". whatever the first line is, the error repeat as the same. should I make changes to file and...
13 October 2023 1,655 1 View
Zinc replaces most of the compounds, and the solution contains significantly more zinc, the goal being to recover the zinc for reuse.
04 August 2023 3,334 0 View
I have seen in many studies, oxygen is used as the plasma gas source to produce ozone in the plasma chamber. But my first question is, are there any advantages if ozone is directly used as the...
03 August 2023 9,035 2 View
In international standards (ASHTO, FHWA, etc.) and many reports of engineering companies, for deep underground structures (deeper than 30 meters), when PGA is transferred to the depth, a reduction...
19 May 2023 738 0 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I am currently investigating the cytotoxicity of a series of herbal extracts, and like many studies, I have been using the MTT assay to evaluate cell viability. However, I am encountering a...
31 July 2024 193 4 View
I did an MTT assay with 2 drugs (Piroxicam and Sulfameter) when I got my results for the 12,24,48,72 hours of incubation with my drug at the following concentrations [ug/ml]...
26 July 2024 7,511 3 View
Hello, I want to ask regarding exosome protein quantification method. Is it necessary to lyse exosomes before measuring their protein contents using BCA assay kit? Or can I just straight away...
23 July 2024 4,603 4 View
Hello, colleagues! There is commenting open for new upcoming edition of USP 1033. Validation target acceptance criteria is now different from what it used to be and it doesn't include Cpm....
23 July 2024 7,292 3 View
Despite not having cells in the media, I am getting purple color. I have tried many troubleshooting methods, varying media types, and even different MTTs from different companies to figure out the...
21 July 2024 9,914 1 View
What cell proliferation assay do you suggest? cyQUANT from thermo Fischer has good reviews, a lot of good articles for the kit from Abcam. and Promega kits look easy and straight forward.
21 July 2024 1,554 2 View
I am extracting proteins from plant samples and am currently attempting to use the TCA/Acetone extraction method. I am having trouble resolubilizing my sample after drying the pellet. I collected...
15 July 2024 9,025 3 View