I need dataset for recommandation system?

Hi every body I need to collect datasets from different source to work on recommendation system algorithms, could you advise me how collect data?

27 February 2021 7,063 7 View

Sample modeling in ABAQUS software and its relationship with the real world?

About crack modeling in ABAQUS: Mode I (pure-tensile cracking) and Mode II (pure-shear cracking), these assumptions may be accruing in theoretical approach (ABAQUS) not practical in Lab....

19 February 2021 6,156 1 View

What is a good software for non-linear curve fitting rather than Excel and Lab Fit?

I am looking for good software for curve fitting. My curve equation is non-linear. I used Lab Fit and Excel software but could not find the curve equation with good accuracy. What software do you...

20 January 2021 5,554 13 View

How can I plot MODIS HDF-EOS grid images into geographic coordinates (lat-lon)?

I'm analyzing Madis products (MCD64A1), when I finish my analysis, I have a 2400X2400 matrix that I want to draw, but since the original image was in sine design, when using From imagesc, draws...

14 January 2021 6,534 2 View

Geographic location of MCD64A1 images in hdf format"?

Is the lat, lon (geographical location) extracted from the MCD64A1 images in hdf format accurate, or should they be converted to the exact location because they are sinusoidal?

31 December 2020 4,988 3 View

Data set for customer baseline load (CBL) estimation?

23 December 2020 6,739 2 View

Output of rankMANOVA in R?

Dear all, I am working on data including two responses (count data, relatively correlated~R2=0.4, non-normal distribution, and violated the homogeneity of covariance) and three independent...

26 November 2020 711 3 View

As an EFL/ESL English teacher has your professional identity changed in any way during this time of virtual teaching?

Since teacher professional identity is ever changing and not fluid, do you feel as an English teacher your identity has in any way changed due to online teaching? Are you the same person when...

14 November 2020 7,788 11 View

For modifying of dna probe, which end of strand dna is better to modify, the end of 3 pime or 5?

Electrochemical microrna biosensor

01 November 2020 5,271 1 View

How has the Covid pandemic affected your classes as an English teacher? Have you been forced to switch to online teaching instead of f2f teaching?

I am interested to know how has English teachers' working conditions changed due to the pandemic and how they are coping with the new situation.

12 October 2020 1,803 1 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View

EMSA troubleshooting. Protein-DNA complex not migrating, cold probe lane with multiple bands. Can anyone help?

I'm running an EMSA to show the DNA binding activity of recombinant proteins versus the Wild type. Previous assays run by my research group using a different test system show the protein-DNA...

24 February 2021 8,325 1 View

What is the proper in vitro culturing method to get information about the activation of innate immune cells by VLPs?

Dear All, I am interested in studying the response of immune cells against virus-like particles (VLPs). I used the PBMC in vitro assay and treat it with the VLP using PHA as a positive ctrl while...

16 February 2021 1,337 2 View

Why does flow deformation occur at the test line and control line on nitrocellulose membranes (LFIA)?

I use 1% BSA in 10 mM phosphate buffer (pH 7.4) solution when striping my nitrocellulose membranes with antigens and antibodies. However, I always observe deformed flow when my sample or running...

16 February 2021 4,224 3 View

Can the viability assays (ex: MTT/CCK-8) measure cell proliferation? or are they solely viability assays?

I want to conduct a proliferation assay to test a chemotherapy combination on a cancer cell line. At first, I thought about the MTT assay. But now, all sources are saying that these do not measure...

14 February 2021 3,142 7 View

GST assay in microplate?

I'd like to know if anyone can provide me with a protocol for traditional GST enzymatic assay (with GSH and CDNB as substrates) in a 96 well microplate, for cell line and/or C.elegans...

11 February 2021 1,328 3 View

What lysis buffer is compatible with Bradford assay?

Hello! I am planning to use Bradford assay to measure protein content in PBMCs lysates. The problem is that Bradford assay doesn't seem to be compatible with Tris-based buffers or SDS. Are there...

10 February 2021 1,797 5 View

For PC-3 cell line. How long before irradiation do I have to synchronize my cells to G0/G1?

I am trying to do a clonogenic assay for PC-3 after irradiation. I have to synchronize my cells by serum starvation but there's a optimal time I have to do that before irradiation. been looking in...

09 February 2021 9,480 1 View

What is best technique to quantify necrotic cell death of specific cell populations?

I currently using FACS (Aurora Cytek) to quantify necrotic Gr1+ cell death from the spleens of immune competent mice. I target Gr1+ cells in the spleens of tumor-bearing mice using a photodynamic...

08 February 2021 733 6 View