I need dataset for recommandation system?

Hi every body I need to collect datasets from different source to work on recommendation system algorithms, could you advise me how collect data?

27 February 2021 7,063 7 View

How can I plot MODIS HDF-EOS grid images into geographic coordinates (lat-lon)?

I'm analyzing Madis products (MCD64A1), when I finish my analysis, I have a 2400X2400 matrix that I want to draw, but since the original image was in sine design, when using From imagesc, draws...

14 January 2021 6,534 2 View

Geographic location of MCD64A1 images in hdf format"?

Is the lat, lon (geographical location) extracted from the MCD64A1 images in hdf format accurate, or should they be converted to the exact location because they are sinusoidal?

31 December 2020 4,988 3 View

Data set for customer baseline load (CBL) estimation?

23 December 2020 6,739 2 View

Output of rankMANOVA in R?

Dear all, I am working on data including two responses (count data, relatively correlated~R2=0.4, non-normal distribution, and violated the homogeneity of covariance) and three independent...

26 November 2020 711 3 View

As an EFL/ESL English teacher has your professional identity changed in any way during this time of virtual teaching?

Since teacher professional identity is ever changing and not fluid, do you feel as an English teacher your identity has in any way changed due to online teaching? Are you the same person when...

14 November 2020 7,788 11 View

For modifying of dna probe, which end of strand dna is better to modify, the end of 3 pime or 5?

Electrochemical microrna biosensor

01 November 2020 5,271 1 View

How has the Covid pandemic affected your classes as an English teacher? Have you been forced to switch to online teaching instead of f2f teaching?

I am interested to know how has English teachers' working conditions changed due to the pandemic and how they are coping with the new situation.

12 October 2020 1,803 1 View

What is the effect of Ascorbic acid in the synthesis of Au nanorods?

I prepared seed solution with HAuCl, CTAB, and NaBH4, after this growth solution with HAuCl4, CTAB, AgNO3, and Ascorbic acid. In the end step, I added Ascorbic acid in growth solution and solution...

17 August 2020 7,506 3 View

Is it possible to image transparent tissue using dark-field microscopy?

I was wondering whether it is possible to image transparent tissue like cornea, with dark-field imaging technology? I'd appreciate any help :)!

29 October 2019 8,301 1 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

Can bacterial suspension be pipetted directly onto petri dish and agar poured onto it?

Hi, recently I encountered a protocol for plating which was not the usual method I have been practicing (pour LB agar onto plate, allow it to solidify, pipette bacterial suspension onto plate,...

28 February 2021 7,292 3 View

How can I assign a velocity along one direction to CO2 molecules in a molecular dynamics simulation using GROMACS?

Dear all, I am trying to perform a MD simulation about the interaction between CO2 and a polymer. I create my model, solvate it and perform the NVT and NPT equilibration. Now I am not able to...

26 February 2021 10,014 3 View

What is the best surface to culture cells isolated from a skin biopsy?

Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...

26 February 2021 6,151 2 View

Difficulty in cloning of 1kb gene into vector?

I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...

25 February 2021 3,221 7 View

Recalcitrant seed short-mid storage method?

Dear peer scientists, Ultradry storage is not suitable for recalcitrant seeds. However, theoretically, if we can stabilize the structure of the subcellular membrane, as well as glassify the...

25 February 2021 2,442 1 View

Can cells be reused after using WST 8/ CCK 8 from Sigma Aldrich?

Hi all, Can cells be reused after I have seed them onto 96 well plates and test them with cell counting kit at OD450 (CCK8) at 12 hours, wash them with PBS x3 and add new media and let them...

24 February 2021 5,072 1 View