Hello Zohreh Haghri,

I would recommend the use of optiMEM which is a media with reduced serum to dilute lipofectamine and DNA before complex formation.

OptiMEM allows for reduction in serum by 50% with no change in growth rate and or morphology. So, OptiMEM would be an appropriate medium when using lipofectamine during transfection.

However, you cannot keep your cells in OptiMEM for longer duration. Preferably 5-6 hours in OptiMEM would be appropriate.

Best.

You can try PolyPlus transfection reagents. Polyplus Interferin reagent works with serum-free DMEM.

i would use Opti-MEM, always give me a good transfection results

Thank you every one😊