I need dataset for recommandation system?

Hi every body I need to collect datasets from different source to work on recommendation system algorithms, could you advise me how collect data?

27 February 2021 7,063 7 View

How can I plot MODIS HDF-EOS grid images into geographic coordinates (lat-lon)?

I'm analyzing Madis products (MCD64A1), when I finish my analysis, I have a 2400X2400 matrix that I want to draw, but since the original image was in sine design, when using From imagesc, draws...

14 January 2021 6,534 2 View

Geographic location of MCD64A1 images in hdf format"?

Is the lat, lon (geographical location) extracted from the MCD64A1 images in hdf format accurate, or should they be converted to the exact location because they are sinusoidal?

31 December 2020 4,988 3 View

Data set for customer baseline load (CBL) estimation?

23 December 2020 6,739 2 View

Output of rankMANOVA in R?

Dear all, I am working on data including two responses (count data, relatively correlated~R2=0.4, non-normal distribution, and violated the homogeneity of covariance) and three independent...

26 November 2020 711 3 View

As an EFL/ESL English teacher has your professional identity changed in any way during this time of virtual teaching?

Since teacher professional identity is ever changing and not fluid, do you feel as an English teacher your identity has in any way changed due to online teaching? Are you the same person when...

14 November 2020 7,788 11 View

For modifying of dna probe, which end of strand dna is better to modify, the end of 3 pime or 5?

Electrochemical microrna biosensor

01 November 2020 5,271 1 View

How has the Covid pandemic affected your classes as an English teacher? Have you been forced to switch to online teaching instead of f2f teaching?

I am interested to know how has English teachers' working conditions changed due to the pandemic and how they are coping with the new situation.

12 October 2020 1,803 1 View

What is the effect of Ascorbic acid in the synthesis of Au nanorods?

I prepared seed solution with HAuCl, CTAB, and NaBH4, after this growth solution with HAuCl4, CTAB, AgNO3, and Ascorbic acid. In the end step, I added Ascorbic acid in growth solution and solution...

17 August 2020 7,506 3 View

Is it possible to image transparent tissue using dark-field microscopy?

I was wondering whether it is possible to image transparent tissue like cornea, with dark-field imaging technology? I'd appreciate any help :)!

29 October 2019 8,301 1 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View

What is a good reagent for lentiviral plasmid transfection in HeLa cells?

I have been using Lipofectamine 2000 but was hoping there may be a better protocol or one for something like Lipofectamine PLUS reagent.

24 February 2021 1,633 4 View

Why the cells die after stable transfection?

I tried stable transfection in the U87 cell line with the sgRNA-Cas9 All-in-One lentivector. Cells were cultured in a 6-well plate, 4ug plasmid was transfected after 24hs when the cell confluence...

22 February 2021 2,864 4 View

Is there a method for evaluating nanoparticle adhesion to epithelial tissue in vitro?

I want to measure how well nanoparticles can transfect cells based on NP adhesion properties. In the literature I find people do it in animal studies, and epithelial adhesion helps in transfection...

21 February 2021 7,791 3 View

Are exogenous molecules transferred between human cells during doubling ?

Hello RG community, I wonderer whether exogenous molecules (e.g. reporter plasmids, miRNAs or other artificial molecules) are transferred between cultured human cells during their doubling ? So,...

21 February 2021 6,633 3 View

Which transfection reagent is the best for plasmid transfection of MDA-MB-231 cell line, Lipofectamine based or other reagent suggestion?

I try to transfect MDA MB 231 cell line with a plasmid by PEI, unfortunately, the rate of transfection is low, and now I wanna find and order the best transfection reagent for this cell line and...

19 February 2021 828 6 View