I can not see the bands.

More Haitham E. M. Zaki's questions See All

Can anyone suggest alternatives for ethidium bromide in terms of a non-toxic DNA staining dye?

07 August 2013 1,920 6 View

After making cDNA for RT-PCR, do you think it is better to adjust its concentration? If yes what is a good concentration of cDNA to use?

I would like to check my genes' expression by RT-PCR. cDAN was synthesized from total RNA.

01 February 2013 9,251 8 View

Total RNA Isolation

I have to isolate the Total RNA from a very small amount of plant tissue. So what do you think, which kit is suitable to use to extract the RNA from this small amount?

31 December 2012 6,643 1 View

Does anyone know how to extract the RNA from a very small amount of plant tissues?

RNA Isolation from small amount of leaves and roots.

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Isolation of total RNA from leaves and roots.

I would like to extract the total RNA from a very small amount of leaves and roots in potatoes, what is a suitable kit for this?

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How to make standard plasmid to check the genes expression by using real time PCR?

To check the expression of the genes by real time PCR you may need to make the standard plasmid.

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How to analyze the carotene components in sweet corn seeds?

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Does anyone know how to compare one gene in arabidopsis for example with the potato one?

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Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

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How to regulate SSCP temperature in simple PAGE?

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Why are my bands weird in this Agarose gel?

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Which cell line should we use to perform biological dosimetry experiments?

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Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

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Calculating volume of DNA required from DNA concentration?

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Has anyone used the Illumina ITS1 primer sets?

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Jetty Ramadevi

Hi!

No worries. Too much of expose to UV generally dyes fade away. So better stain it and see you can see you DNA bands as usual.

Upendar Rao Golla

Dear Zaki,

It looks like that the staining dye is getting fade as suggested by Jetty. Better stain for long time or use increased concentration to see at repeated times.

Good Luck....!!!