Can anyone suggest alternatives for ethidium bromide in terms of a non-toxic DNA staining dye?

Can anyone suggest alternatives for ethidium bromide in terms of a non-toxic DNA staining dye?

07 August 2013 1,920 6 View

After making cDNA for RT-PCR, do you think it is better to adjust its concentration? If yes what is a good concentration of cDNA to use?

I would like to check my genes' expression by RT-PCR. cDAN was synthesized from total RNA.

01 February 2013 9,251 8 View

I can not see the bands.

After running the gel and the gel is ready for imaging, I can see the bands very clearly but after a while the bands become unclear. If i wait 5 min I can not see them at all. Does anybody know...

01 February 2013 6,879 2 View

Does anyone know how to extract the RNA from a very small amount of plant tissues?

RNA Isolation from small amount of leaves and roots.

31 December 2012 8,117 2 View

Isolation of total RNA from leaves and roots.

I would like to extract the total RNA from a very small amount of leaves and roots in potatoes, what is a suitable kit for this?

31 December 2012 2,594 0 View

How to make standard plasmid to check the genes expression by using real time PCR?

To check the expression of the genes by real time PCR you may need to make the standard plasmid.

10 November 2012 5,107 3 View

How to analyze the carotene components in sweet corn seeds?

I have done some crosses between corn and sweet corn. I would like to estimate the carotene components on the obtained plants.

10 November 2012 5,963 4 View

Does anyone know how to compare one gene in arabidopsis for example with the potato one?

I am working with potato and I found one of my interested genes in Arabidopsis, what I need now is to find this gene in potato. I have tried NCBI and all of these websites but I could not find it.

10 November 2012 1,595 3 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

No title

02 March 2021 3,060 3 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

How to remove fatty material from plant extract?

The resultant solid was partitioned three times with 250 ml of petroleum ether (40-60/c) to remove any fatty material and evaporated to dryness.

28 February 2021 9,464 2 View