How to analyze the carotene components in sweet corn seeds?

More Haitham E. M. Zaki's questions See All

Can anyone suggest alternatives for ethidium bromide in terms of a non-toxic DNA staining dye?

07 August 2013 2,015 6 View

After making cDNA for RT-PCR, do you think it is better to adjust its concentration? If yes what is a good concentration of cDNA to use?

I would like to check my genes' expression by RT-PCR. cDAN was synthesized from total RNA.

01 February 2013 9,361 8 View

I can not see the bands.

After running the gel and the gel is ready for imaging, I can see the bands very clearly but after a while the bands become unclear. If i wait 5 min I can not see them at all. Does anybody know...

01 February 2013 6,974 2 View

Total RNA Isolation

I have to isolate the Total RNA from a very small amount of plant tissue. So what do you think, which kit is suitable to use to extract the RNA from this small amount?

31 December 2012 6,745 1 View

Does anyone know how to extract the RNA from a very small amount of plant tissues?

RNA Isolation from small amount of leaves and roots.

31 December 2012 8,228 2 View

Isolation of total RNA from leaves and roots.

I would like to extract the total RNA from a very small amount of leaves and roots in potatoes, what is a suitable kit for this?

31 December 2012 2,686 0 View

How to make standard plasmid to check the genes expression by using real time PCR?

To check the expression of the genes by real time PCR you may need to make the standard plasmid.

10 November 2012 5,240 3 View

Does anyone know how to compare one gene in arabidopsis for example with the potato one?

I am working with potato and I found one of my interested genes in Arabidopsis, what I need now is to find this gene in potato. I have tried NCBI and all of these websites but I could not find it.

10 November 2012 1,697 3 View

Senescence-associated beta galactosidase staining is False Positive in control group?

Hi guys If anyone is currently working on aging cells, you guys would like to give me some advice. I'm testing against biomarker (SA-beta-Gal), I encountered a false positive in the control group...

02 August 2024 6,735 1 View

How am I supposed to find justifications for the unusual fatty acid profile in broiler diet?

I've obtained a fatty acid profile of broiler diet comprised of SFA 48 to 93%, MUFA (22 to 40%), PUFA (non determined to 10.4%. The PUFA composition is mainly n-6 and almost no value for n-3. The...

08 July 2024 9,165 4 View

What is the best part of a leaf to study the anatomy of monocot and dicot plants?

The first 1/3 of the leaf (near the stem), the middle 1/3 of the leaf, the last 1/3 of the leaf Monocot: Corn Dicot: bean

28 June 2024 2,607 4 View

What type of paraffin is recommended for preparing samples for cutting with a microtome?

Sample type: Corn leaves and stem Bean leaves and stem

22 June 2024 2,082 3 View

Beta galactosidase assay in milk?

I am planning to measure the the activity of beta galactosidase enzyme using a standard kit to detect glucose production. The kit reagent is a mixture including horseradish peroxidase and...

12 June 2024 7,331 1 View

Beta cyclodextrin complex characterization methods?

Which is the best method for the characterization of beta cyclodextrin complex formation with guest molecule in solution form and in solid form ?

11 June 2024 6,719 0 View

In GC MS Phytochemical analysis and screening for NIST library, some compounds are repeated at different retention time. Should I include them all?

Should I include them all in a table or should I exclude them for publication purpose

06 June 2024 5,489 5 View

How to convert mIU/mL of beta galactosidase enzyme to pg/ml? I have used ELISA to determine beta gal concentration in mIU/ mL in human serum samples?

We have to report the concentration in pg/ml, the ELISA kit provider did not reveal the specific activity of the enzyme. In that case how to convert the units?

05 June 2024 2,173 5 View

Has anyone used Dinitroaniline herbicides to double the chromosomes of haploid corn?

Greetings! Has anyone used Dinitroaniline herbicides to double the chromosomes of haploid corn? If did, which one and the details, please. Thank you very much!

29 May 2024 9,836 1 View

How Can I Understand the Different Results from MacConkey Agar and β-Galactosidase Assays in a LexA-Based Bacterial-Two Hybrid System?

I'm conducting protein-protein interaction studies using a LexA-based Bacterial-Two Hybrid system with E. coli reporter strains. We employ MacConkey agar and β-galactosidase assays to evaluate the...

26 May 2024 4,647 0 View

M. Ángeles Ramírez-Cisneros

There is an article than could help you: Journal of Biomedicine and Biotechnology

Volume 2012, Article ID 524019, 10 pages

doi:10.1155/2012/524019 good luck!