Can anyone suggest alternatives for ethidium bromide in terms of a non-toxic DNA staining dye?

Can anyone suggest alternatives for ethidium bromide in terms of a non-toxic DNA staining dye?

07 August 2013 1,920 6 View

After making cDNA for RT-PCR, do you think it is better to adjust its concentration? If yes what is a good concentration of cDNA to use?

I would like to check my genes' expression by RT-PCR. cDAN was synthesized from total RNA.

01 February 2013 9,251 8 View

I can not see the bands.

After running the gel and the gel is ready for imaging, I can see the bands very clearly but after a while the bands become unclear. If i wait 5 min I can not see them at all. Does anybody know...

01 February 2013 6,879 2 View

Total RNA Isolation

I have to isolate the Total RNA from a very small amount of plant tissue. So what do you think, which kit is suitable to use to extract the RNA from this small amount?

31 December 2012 6,643 1 View

Does anyone know how to extract the RNA from a very small amount of plant tissues?

RNA Isolation from small amount of leaves and roots.

31 December 2012 8,117 2 View

Isolation of total RNA from leaves and roots.

I would like to extract the total RNA from a very small amount of leaves and roots in potatoes, what is a suitable kit for this?

31 December 2012 2,594 0 View

How to make standard plasmid to check the genes expression by using real time PCR?

To check the expression of the genes by real time PCR you may need to make the standard plasmid.

10 November 2012 5,107 3 View

Does anyone know how to compare one gene in arabidopsis for example with the potato one?

I am working with potato and I found one of my interested genes in Arabidopsis, what I need now is to find this gene in potato. I have tried NCBI and all of these websites but I could not find it.

10 November 2012 1,595 3 View

How can plant phytochemistry be included in discussions about whole plant traits?

I am using the concept of plant functional traits (Cornellisen et al., 2003) in a paper. Considering descriptions for whole plant traits (life history; life form; growth form; plant height;...

24 February 2021 6,831 5 View

Help with Amyloid beta detection protocol (western-blotting)?

Hi everyone, I am a first year PhD student working on amyloidosis. I would like some tips and tricks to crack efficiently my current nightmare, i.e. the western blotting of the Amyloid beta and...

11 February 2021 2,513 3 View

Why do high doses of humic substances inhibit the growth of corn roots? Is there a toxicity effect?

Length, volume, surface area and dry mass of corn roots treated with humic substances increased up to 13 liters per hectare and decreased with higher doses.

02 February 2021 2,190 4 View

Does increasing antibiotic concentration also increase plasmid production in DH5a E.coli?

Dear all, I am propagating some plasmids with a human gene and an ampicillin resistance gene. My logic is that if ampicillin concentration will be increased, the concentration of plasmid (pSF)...

14 January 2021 779 3 View

Is corn fiber hydrolysis possible without the use of enzymes?

I dont have access to the needed enzymes for CF hydrolysis, so I'm wondering whether I can do the hydrolysis solely with the use of acids? Has it ever been done before?

08 January 2021 1,243 3 View

How can I reduce the viscosity of corn oil used as vehicle for administration in animal experiments?

Dear all, I am using corn oil as vehicle for subcutaneous injection and I want to reduce the viscosity of the vehicle (corn oil) to enhance the delivery of the main compound of interest....

18 December 2020 1,773 6 View

Why nitrocefin is preferred over CENTA?

The chromogenic beta-lactamase substrate CENTA is at least five times cheaper than the other famous substrate nitrocefin and can be used with most enzymes, still, nitrocefin is preferred. Any...

17 December 2020 3,735 2 View

Are there any alternatives to Corning's Transwell inserts for Air Liquid Interface culture?

There is apparently a worldwide shortage (due to COVID) of these 0.4micron pore, polystyrene membrane transwells...Are there any companies that make suitable, good quality alternates as we are...

09 December 2020 6,771 2 View

Greiner Bio or Corning Costar plates for ELISA?

09 December 2020 237 2 View

How do you confirm your beta receptor antagonist is WORKING before you implement it in a big experiment?

I want to use a beta blocker in vivo but first I want to ensure the drug is working and for how long the effects last after the drug is withdrawn. I'm thinking I can administer the drug and then...

06 December 2020 5,179 1 View