I would like to extract the total RNA from a very small amount of leaves and roots in potatoes, what is a suitable kit for this?
Can anyone suggest alternatives for ethidium bromide in terms of a non-toxic DNA staining dye?
07 August 2013 2,015 6 View
I would like to check my genes' expression by RT-PCR. cDAN was synthesized from total RNA.
01 February 2013 9,361 8 View
After running the gel and the gel is ready for imaging, I can see the bands very clearly but after a while the bands become unclear. If i wait 5 min I can not see them at all. Does anybody know...
01 February 2013 6,974 2 View
I have to isolate the Total RNA from a very small amount of plant tissue. So what do you think, which kit is suitable to use to extract the RNA from this small amount?
31 December 2012 6,745 1 View
RNA Isolation from small amount of leaves and roots.
31 December 2012 8,228 2 View
To check the expression of the genes by real time PCR you may need to make the standard plasmid.
10 November 2012 5,240 3 View
I have done some crosses between corn and sweet corn. I would like to estimate the carotene components on the obtained plants.
10 November 2012 6,054 4 View
I am working with potato and I found one of my interested genes in Arabidopsis, what I need now is to find this gene in potato. I have tried NCBI and all of these websites but I could not find it.
10 November 2012 1,697 3 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
10 August 2024 8,539 5 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...
06 August 2024 807 3 View
I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...
06 August 2024 1,367 3 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
HOW CAN I WRITE A CODE TO USE THE WAVENET TRANSFORM AS A FEATURE EXTRACTION METHOD INSTEAD OF DWT IN MATLAB?
03 August 2024 7,829 0 View