I would like to extract the total RNA from a very small amount of leaves and roots in potatoes, what is a suitable kit for this?
Can anyone suggest alternatives for ethidium bromide in terms of a non-toxic DNA staining dye?
07 August 2013 1,920 6 View
I would like to check my genes' expression by RT-PCR. cDAN was synthesized from total RNA.
01 February 2013 9,251 8 View
After running the gel and the gel is ready for imaging, I can see the bands very clearly but after a while the bands become unclear. If i wait 5 min I can not see them at all. Does anybody know...
01 February 2013 6,879 2 View
I have to isolate the Total RNA from a very small amount of plant tissue. So what do you think, which kit is suitable to use to extract the RNA from this small amount?
31 December 2012 6,643 1 View
RNA Isolation from small amount of leaves and roots.
31 December 2012 8,117 2 View
To check the expression of the genes by real time PCR you may need to make the standard plasmid.
10 November 2012 5,107 3 View
I have done some crosses between corn and sweet corn. I would like to estimate the carotene components on the obtained plants.
10 November 2012 5,963 4 View
I am working with potato and I found one of my interested genes in Arabidopsis, what I need now is to find this gene in potato. I have tried NCBI and all of these websites but I could not find it.
10 November 2012 1,595 3 View
dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...
03 March 2021 6,994 5 View
Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue
02 March 2021 5,433 5 View
I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...
28 February 2021 4,949 3 View
I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...
27 February 2021 356 6 View
Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...
27 February 2021 9,233 3 View
Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...
25 February 2021 1,660 3 View
Warm greetings! My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology focusing on Human papillomavirus). At this stage, I have almost completed a clinical sample (Cervical swabs)...
25 February 2021 3,612 3 View
Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...
24 February 2021 5,749 3 View
I have synthesised cDNA from 500ng/ul of RNA. The concentration of the cDNA Is about 5000ng/ul. Should I dilute my cDNA or just use it as it is for my qPCR? I usually use 2ul of cDNA for a 20ul...
24 February 2021 8,618 3 View
I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the...
24 February 2021 3,623 3 View