Lipids used are soy lecithin and cholesterol in weight ratios of 60:40, 75:25 & 80:20mg respectively. Solvent used is chloroform 10ml. Method is thin film hydration. Hydration medium is 10 ml of PB pH 7.4. Hydration carried out at 60 oC. Sonicated using a probe sonicator at 40% amplitude for 15 minutes with 10 sec on and 10 sec off cycles. Stored in refrigerator overnight and zeta potential, Average particle size and PDI measured the next day. PDI was fond to be 0.25-0.38.
Can you give me suggestions about how to obtain vesicles in 150-250 nm size range using probe sonicator. And what should be the sonication parameters for my required size range.
First find out the phase transition temperature of your lipid at that temperature you have to do sonication.
Cholesterol are using for liposome rigidity so if your amplitude will be high then it will disrupt your liposome. set your amplitude upto 35% for 5-7 min with 50 sec on and 10 sec off time.
And also extrude your liposome temperature by polycarbonic membrane.
Thank you.
Hi,
It is very difficult to control size of your vesicles using any type of sonication. Why you don't use filtration or extrusion with precise pore sizes
instead?
I am trying to procure a mini extruder for extrusion purpose. But at the moment I don't have an extrusion system. I have tried vacuum filtration using 0.2 micron pore filter. But he pores get clogged which results in loss of maximum vesicles.
It may be useful to try a pre-filtration with a larger (for example 0.45 micron) pore size to reduce the biggest vesicles first, and avoid clogging the 0.2 micron pores completely. You may also need several passes for better homogeneity.
Poster Multi-Parameter Characterization of Liposomes by Nanoparticl...
T&T Scientific make disposable, single-use mini-extruders (NanoSizer) that are extremely easy to use when sizing liposomes. The NanoSizer extruders are available in multiple pore-sizes including 50nm, 100nm, 200nm, and 400nm, and they will even send free samples for you to try if you contact them!
https://www.ttscientific.com
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