I have prepared prednislone loaded liposomes. Prednisolone being incorporated in the lipid layer, how to separate free drug from liposome suspension to carry out entrapment efficiency studies? I do not have an ultracentrifuge at my disposal. Can you please suggest me alternative method for separating unentrapped drug which has poor aqueous solubility?
Prednisolone is freely soluble in water, so there is very limited scope to get unsoluble drug.
I do agree with Alireza!
With a little consideration you can prepare your own setup with 3 or 4 parallel chambers separated with suitable membranes.
The prednisolone I have is not a salt. Hence its solubility is 223 mg/L. It is prednisolone BP.
Soluble form is prednisolone sodium phosphate.
The best way is dialysis or reverse bag dialysis method.
Or u can use gel filtration method (refer article: Enhancement in antifungal activity of eugenol in immunosuppressed rats through lipid nanocarriers, Anuj garg 2011, Colloids and surfaces:B)?
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