As soon as bFGF arrives, add 1mg BSA/PBS to make 2~4-fold dilution to prevent loss of bFGF molecules as a carrier in the solution. Make rather smaller aliquot in small eppen tubes. Store them with a proper labeling at -70C until use. Once you thawed and diluted bFGF for your preferred concentration to be added to your final culture medium, throw the remaining bFGF away. Only this can provide consistent responses from the treated cells. DO NOT JUST STORE AT FRIDGE without any carrier protein!!!
As soon as bFGF arrives, add 1mg BSA/PBS to make 2~4-fold dilution to prevent loss of bFGF molecules as a carrier in the solution. Make rather smaller aliquot in small eppen tubes. Store them with a proper labeling at -70C until use. Once you thawed and diluted bFGF for your preferred concentration to be added to your final culture medium, throw the remaining bFGF away. Only this can provide consistent responses from the treated cells. DO NOT JUST STORE AT FRIDGE without any carrier protein!!!
I second Sang Ho Lee. You should immediately aliquot the bFGF in BSA/PBS solution and store it at -30 or -80. Make sure to use up the full aliquot in one go. Freezing and thawing effects the stability of bFGF. I preferred to make a 1:20-1:50 dilution and made small aliquots of it. If stored properly, it should give you consistent results.
please, bfgf from peprotech according to the data sheet of the product it will be Reconstituted in 5mM Tris, pH 7.6, to a concentration of 0.1-1.0 mg/ml !
Nehal Ghoneim Hi Nehal, I have been confused about the same thing too. So, I got in touch with Peprotech and they recommended to only use Tris for the initial reconstitution. I think its related to their tests on the protein stability. Following you can dilute in PBS/BSA for long term storage.