Christian Sebald

4 Questions 8 Answers 0 Followers

Questions related from Christian Sebald

I am looking for the stability of bFGF at 4°C in PBS or cell culture media (a-MEM)?

I am looking for the stability of bFGF at 4°C in PBS or cell culture media (a-MEM). Any information?

08 August 2017 8,511 6 View

FACS or ELISA - what would be the best way to determine the cytotoxic potential of expanded T cells (esp. TILs) ?

We want a rapid method to determine the cytotoxic potential, refering to the level of IFN-g, TNF-a, Perforin, Granzyme, without using an assay for direct cytotoxicity.

05 May 2017 2,219 8 View

Can someone send me a LDH Assay protocol without using a comercial kit?

We want to dtermine the cytotoxicity of NK cells without the use of a comercial kit.

05 May 2017 2,924 5 View

Looking for vital staining non fluorescent dye for the staining of NK cells in vitro. Any suggestions?

Our aim is to visualize the interaction of NK cells with an adherent cancer target cell. We want to stain the NK cells with an non toxic, non fluorescent vital stain to visualize the interaction...

05 May 2017 3,408 1 View