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Questions related from Nehal Ghoneim
Kindly , I have human ASCs and BM-MSCs cell lines from ATCC. They mentioned on the website that they are cultured on mesenchymal stem cells media ! However, I have on my lab DMEM low glucose...
11 November 2018 8,947 4 View
I am confused about the difference between the three components and which is suitable for cell culture as I bought the first one and it is not written if suitable for cell culture or not...
09 September 2018 8,125 0 View
Kindly I need to coculture between neuroblastoma cell line (which I culture in DMEM low glucose medium with 10 % FBS) and other cell line that needs to be cultured in DMEM Megacell medium , So I...
08 August 2018 4,975 0 View
How can I set ascale bar using image j soft ware for already taken images with leica inverted microscope. I have already followed the following steps: 1) Open the image using image j 2)From...
04 April 2018 6,167 0 View
Please, I don't know which is better Bio rad Chemi DOC xrs+ or Azure Biosystems™ c280 for protein and DNA gel applications !
04 April 2018 3,527 5 View
I am doing plasmid purification for about 20 Microgram yield , I wonder which is best Qiagen mini prep or promega ? and is the expiry date of Qiagen kit 1 year from opening the kit or the...
04 April 2018 525 3 View
Kindly I need to do transfection for canecr cell lines by Crispr cas 9 from santa cruiz (downregulation and overexpression) , I wonder if cuvette 0.2 will work with it as I don't have 0.4 and the...
03 March 2018 269 2 View
I am trying to do transfection of SNU 449 hepatocellular carcinoma cells by GFP plasmids using Bio Rad micropulser electroporator ! according to its protocol it nables safe and reproducible...
03 March 2018 3,185 4 View
I am trying to transfect SNU449 cell line by GFP plasmids using genepulserX Bio rad electroporator. I wonder if I can use .4 cm cuvette and voltage needed ! and what is the cell size of SNU449 !
03 March 2018 2,060 4 View
