Is there any procedure or an equation for converting total carbon to organic carbon or any equation that helps to obtain the value?

I have analysed percentage carbon (total) using CHNS Elemental Analyzer for the forest soils. Now, I would like to obtain SOC content from the obtained value for which I am looking for a procedure...

29 January 2021 5,551 8 View

How to enter a variable with multiple selections as response in SPSS software?

Hello I wanted to ask how can I enter a variable in SPSS with the option of multiple responses for a given variable? For example: If question is "What is your role?" Answer Choices: (A) Positive...

08 January 2021 5,741 4 View

Anyone knowing any software for scientific graphs plotting?

I need some help

03 December 2020 6,794 6 View

Any good references for writing a master thesis for new business idea?

I am a Masters's student and I want to write my Master Thesis for a new business idea/startup. I have been searching for references that I can use as a guide to starting my thesis proposal and...

18 October 2020 9,820 2 View

Crustal Modelling by Gravity and Magnetic Data?

Dear Researchers, What are the Convenient methods for Crustal Modelling using Gravity and Magnetic Data?

20 September 2020 8,977 3 View

What is the most reliable Index for researchers in 2020. (1). Google Scholar (2). Scopus (3). Publons (4). RG ?

Dear teachers and fellows, what do you think about various indicators and which indicator is more reliable to evaluate the impact of scientific research? The same scholar has a different...

01 February 2020 8,141 65 View

Why nano-cutting fluids are known as green or environmental friendly fluids?

Recently traditional cutting fluids are gradually being replaced by the nanofluid due to growing need of green manufacturing and environmentally friendly process. ref: Experimental Study of Heat...

19 September 2019 1,128 4 View

Can anyone provide me the literature/method for Quinalphos pesticide determination by UV-Vis Spectrophotometer?

Quinalphos: An Organophosphate pesticide

17 July 2019 7,304 5 View

What is the most suitable filter vial for amino acids in methanol for running in uPLC?

I am running amino acids in uPLC. The aa are extracted from bilogical fluids using methanol. Then, it is recomanded to filter the sample before runing in uPLC. However, the are so many types of...

24 June 2019 7,476 4 View

Can we do a semi-quantitative protein analysis using SDS-PAGE, by UniProtKB protein database?

I have done SDS-PAGE for serum proteins (NP-protein interaction) and i am getting quite a good number of bands, now i want to probably identify the protein that are associated with the NPs, can i...

08 April 2019 5,240 3 View

What is the minimum number of exosomes/ml to have a good yield of total RNA for Illumina sequencing?

I am using IZON qEV2 columns to isolate exosomes from 100 ml of cancer cell conditioned medium. in NTA analysis I usually see concentration of 2x10^9 particles/ml. But when I proceed with this...

20 February 2021 6,879 3 View

Should the AUC in a concentration-time graph of a subcutaneous implant be roughly the same for poeple?

I have 2 concentration time curves of a subcut drug implant. the first group has a peak around the 50 day mark where as the second group does not. Both groups received the same implant by the same...

10 February 2021 2,305 3 View

How to choose the best segmentation model using the area under the precision recall curve, IOU and Dice metrics?

I am using several U-Net variants for a medical image segmentation task. I get the following values for the performance measures including Dice, IOU, Area under receiver-operating characteristic...

27 December 2020 7,273 3 View

Has anyone used Isolate II RNA mini kit for isolation miRNA from exosomes?

I will be isolating exosomes from plasma with Total Exosome Isolation Kit (Invitrogen). Will Isolate II RNA mini kit (Bioline) be appropriate for miRNA isolation from obtained exosomes? The...

26 November 2020 9,503 5 View

Which ABs concentration for EVs immuno-staining?

Hi all, I have to do a phenotyping study on cell culture derived EVs, in particular I should stain them using primary labelled ABs anti-EVs markers before proceeding to analyze them through...

25 November 2020 4,601 1 View

What is the best RNA isolation method from exosomes of serum samples ?

12 November 2020 9,331 7 View

Exosome quantification by Elisa based method ?

Hi, I'm working on exosomes isolated from blood samples and cell culture supernatant. I'm wondering if I could quantify exosomes based on Elisa Method coated with exo Ab (CD63 / CD9/C81...) My...

11 November 2020 664 3 View

How to properly collect/store exosomes (isolated from conditioned culture media) for a later proteomic and/or metabolomic profiling?

I want to ask how to properly collect and store exosomes just after their isolation from conditioned culture media, for subsequent proteomic and/or metabolomic profiling ?? Should we make snap...

06 November 2020 5,201 3 View

Could anyone recommend a kit to isolate exosome from cell culture supernatant?

Hello, I'm working with immortalized HUVECs low and I'm trying to study their exosomal RNA contents. Could anyone recommend an effective kit to isolate exosomes from cell culture supernatant?...

31 October 2020 4,252 5 View

How to extract extracellular vesicles from cell lines?

Recently, there was a problem with my qpcr results. I want to try more methods of extracting EV from the cell medium. At present, I am using Invitrogen's kit(Cat4478359) to extract exosomes. Can...

28 October 2020 2,961 3 View