I´m looking for a hypoosmótic model in mice, could somebody help me?
I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...
02 March 2021 4,443 2 View
My baseline increases without any explanation, it is only fixed by changing the solution B, will it be the column? Reagents? Bomb? Detector? Solution A: H3PO4 0.01% IN WATER Solution B: H3PO4...
21 February 2021 5,656 3 View
I need to modify the blood pressure in a monkey while is awake in the recording chamber, and I am planing on provide a drug for that in the food or water used for reward... any advise? Thank you
08 February 2021 9,900 1 View
Hello everyone, two months ago I did a transfection of HEKs cells to insert a miR using the JetPei reagent. On that occasion, the transfection was highly efficient and the cells were in perfect...
03 February 2021 1,296 6 View
Hi everyone, I have been culturing HEK293T cells that have been engineered to overexpress the mechanosensitive protein Piezo1. However, I am finding it very difficult to keep them in culture -...
02 February 2021 7,892 4 View
Even following the instruction of Karnovsky and Roots (1964), I still do not know how long I should fix the larvae and in what fixative. Could anyone help me? Thank you!
14 January 2021 385 2 View
07 January 2021 4,802 2 View
Hello, everyone: I've been reading some old and new papers on how to compute beta diversity using incidence or abundance data from several assemblages/communities. For my data, I am interested...
03 January 2021 9,293 1 View
22 November 2020 8,558 9 View
Would it be possible to add -OH functional groups to Bi2Te3 nanoparticles?
07 October 2020 6,560 3 View
Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?
03 March 2021 1,763 2 View
Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...
02 March 2021 4,569 4 View
Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...
02 March 2021 6,865 1 View
Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...
01 March 2021 6,953 1 View
Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...
01 March 2021 9,938 3 View
Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!
28 February 2021 3,951 2 View
In R-studio, there are many commands of Gumbel package. Arguments are also different. I`m asking about the alpha parameter of the Copula which must be greater than 1. If this is the one used to...
25 February 2021 5,229 3 View
Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...
25 February 2021 155 2 View
Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...
24 February 2021 1,825 3 View
Hello, I am currently using a Cas9 knock in system that will be expressed in the liver. To do so STOP codon fl/fl Cas9 KI mice were bred with Albumin-Cre mice to generate heterozgous pups, that...
24 February 2021 5,759 3 View
