Does HPLC grade reagents serve for UHPLC-MS quantification? Even filtered with 0.22 micron pore? Thanks in advance
LC-MS grade solvents are chemically "cleaner" than HPLC grade solvents. A high quality solvent will pose less issues with background MS noise in the long term. Now, it also depends on your application. If you are working at parts per million level, higher quality solvent the better, because you would see less background. However, if your concentrations are high, there is no need to worry too much about noise. You did not mention your application.
Just to add a comment on filtering of solvents in a laboratory. Filtering high quality solvents (e.g. HPLC grade), in a typical laboratory set-up produces a more contaminated solvent than actually present in the original bottle.
I saw a poster by Merck (?) in a conference where they showed that typical deionized water remains "deionized" for approx. 30 min. Filtering deionized water in glass, decreases the resistance from 18 MOhm to 16-17 MOhms very quickly. Within 30 minutes ammonium ions could be traced in water. For research purposes, we do not filter solvents which are already labelled as HPLC grade from Sigma etc.
The HPLC grade solvent is made to have less UV absorbance which is not relevant to the MS. HPLC is the same as UPLC except fir the latter one is done at a high pressure environment because the particle size of the stationarybphase is smaller . The anwer is no difference andbyou can use it
LC-MS grade solvents are chemically "cleaner" than HPLC grade solvents. A high quality solvent will pose less issues with background MS noise in the long term. Now, it also depends on your application. If you are working at parts per million level, higher quality solvent the better, because you would see less background. However, if your concentrations are high, there is no need to worry too much about noise. You did not mention your application.
Just to add a comment on filtering of solvents in a laboratory. Filtering high quality solvents (e.g. HPLC grade), in a typical laboratory set-up produces a more contaminated solvent than actually present in the original bottle.
I saw a poster by Merck (?) in a conference where they showed that typical deionized water remains "deionized" for approx. 30 min. Filtering deionized water in glass, decreases the resistance from 18 MOhm to 16-17 MOhms very quickly. Within 30 minutes ammonium ions could be traced in water. For research purposes, we do not filter solvents which are already labelled as HPLC grade from Sigma etc.
There are solvents specially designated "Chromasolv - tested for UHPLC", by Fluka, for example. Very expensive, but you can't go any lower for fine applications. And yes indeed, if you start filtering your HPLC solvents, or in any way try to improve them, you'll create more harm than good.
Use Riedel-de Haën, Burdick and Jackson, Honeywell any of these brand its same. Surely very costly.. but 100 % reliable.
we have used the HPLC grade solvents like acetonitrile/methanol/meluqie water for making mobile phase in UHPLC and LC-MS and there is no problem. the only thing observed is long life time in the case of LC-MS slowly eluent.
LC-MS solvents undergo several distinct tests to ensure they meet the stringent criteria required for sensitive LC-MS and LC-UV analyses. Some of the most important features are:
- Application-tested for LC-MS using the reserpine test
- Very low level of inorganic and metal ions for high sensitivity and spectral interpretation
- Free of particles and non-volatile compounds to maintain system integrity
- High UV-transmittance for UV-Diode Array applications
- Low gradient baseline even with your own optimized protocols
UHPLC solvents should be filtered to at least 0.1 um to work reliably in a UHPLCMS application. Also, HPLC solvents are not typically checked for MS background. Using an HPLC solvent in a UHPLC application is playing russian Roulette with your instrument, sample and columns.
Hello, Ivan,
just try it out. If your DAD and MS traces look good (no additional peaks or background ions that can't be explained), be happy. After all, it's also a question of money. It's something else when you're working in the field of trace analysis. Then you usually can't get past the better (and unfortunately also much more expensive :(( ) solvent qualities. In our laboratory we use acetonitrile in HPLC quality (analytical + preparative). The water is demineralized water from our company network. The solvents are not filtered. This has been working for us for more than 20 years without any problems. We have LC/MS systems throughout. If something is wrong, we would see it immediately. The water doesn't cause any problems. Years ago I've tested our water and commercial water (from different suppliers) regarding TOC values. Guess who was the best by far.
Regards
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