Dear all, I would like to quantify protein nitrosylation but most ELISA kits are very expensive. I have already quantified the amount of nitrites in cell cultures using the Griess Reaction. Is there a way to quantify nitrites as indirect measurement of protein nitrosylation using the Griess reaction, in hepatic and muscular tissue? Thanks in advance for your help.
It seems quite likely that while performing the Griess reaction both, acidified nitrite and auto oxidized nitric oxide, are transformed into the same diazonium derivate (see Figure 2 in Bryan & Grisham, 2007: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2041919/pdf/nihms28562.pdf). Considering NO´s short life, time course quantification in cellular systems could be tracked by fluorescence and biotin switch (check "Detection of RSNOs in biological systems" in the following article: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4369268/pdf/bph0172-1620.pdf ). Hoping you'll find this information useful
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