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Questions related from Ivan Luzardo-Ocampo
Dear all, we are evaluating the cytotoxic effect of plant extracts on HT-29 and HCT116 colon cancer cells. We have determined the IC50 values for each cell line, however, we want also to test...
10 October 2019 1,331 3 View
Hello, I'll perform flow cytometry (Caspase-1, Annexin-V, PI assay) in differentiated macrophages (from THP-1 monocytes to macrophages using PMA). I usually detach the cells using TryPLE and wait...
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Does HPLC grade reagents serve for UHPLC-MS quantification? Even filtered with 0.22 micron pore? Thanks in advance
02 February 2018 5,979 10 View
Dear all, I'm actually preparing cell culture medium for RAW 264.7 (macrophages). Many of protocols establish a double 0.22 micron filters, but my question is: it is necessary to use two (double)...
09 September 2016 9,483 0 View
Dear all...I'm evaluating inflammation on macrophages (cell line: RAW 264.7) but actually I'm just culturing them. Is it possible to detach them using trypsin? I've read in some articles that...
05 May 2016 8,709 3 View
