How you can define non coding RNA, gene and pseudogene??

More Ratih kusuma Wardani's questions See All

Can anyone help, why my GAPDH band seems not uniform?

I make cDNA from uniform RNA concentration 1 mikrogram/mikroliter, did it have corelation with PCR condition?? or because my RNA have low quality??

09 October 2015 9,671 3 View

Does anybody know a web server that is running Molecular Dynamics (MD)?

I want to do a simulation with Molecular Dynamics but lack the facilities.

11 December 2013 2,850 15 View

Any advice on MDweb warning while checking protein?

I am using MDweb to analyse MD. While checking protein, I have been given some warning about polar acceptor clashes, apolar clashes and ionic negative clashes. Any suggestions?

11 December 2013 2,415 1 View

Protein modelling - can anyone help?

I have to model IRS-I protein and I use quark for my modelling, but because the protein is too large I only use protein partially around 37 AA based from article that this amount has effect in...

11 December 2013 6,922 5 View

Protein modelling - can anyone help?

I need to model the IRS-I protein, but the homology modelling and I tasser result is not good enough, I have planned to use quark, but protein length is too large for quark.

10 November 2013 9,152 9 View

What are some tricks to modelling large proteins using quark?

10 November 2013 1,684 6 View

I am running docking with AutoDock Vina, and I am still confused about how to choose a reliable range for binding affinity?

I have found the best results with a binding affinity of 6,7 kj/mol.

09 October 2013 3,923 1 View

Error using AutoDock 4?

I ran AutoDock 4 via PyRx. I got this error: "AttributeError: Docking instance has no attribute 'ligMol'." How do I solve it?

09 October 2013 2,040 11 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

How to code open-ended questions in SPSS?

Hello all, In SPSS I am going to code 2 open-ended questions. I have already read all the answers and I made a list of the most important categories to which I can code the answers. This question...

02 March 2021 1,757 4 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

How to eliminate Steady state error from Reinforcement learning genetic algorithm code?

The following code (see 1st 2 images attached) is used to produce PID controller values that are designed to control the system (G). The code finds the PID controller values (noted as k) by using...

28 February 2021 6,560 14 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View

How can we simulate an hyperelastic-viscoelastic behavor with Ansys APDL?

I am working on modeling and simulation of biomecanical material behaviour, I have succeded on simulating skin using anisotropic hyperelastic material, on APDL and in our lab's finite element...

28 February 2021 552 3 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

Modeling viscosity using model of Chung?

Hello, As part of simulation of gases mixture and water, I need to calculate viscosity of the fluid components and I am using relationships proposed by Chung et al. (1988). The irony is while I...

25 February 2021 8,053 5 View

Ratih kusuma Wardani

aahh,,thank you very much for your answer....terima kasih banyak atas bantuannya

an on NCBI that gene mention as predicted gene, long non coding RNA,,in ensembl mention that gene is predicted gene, they even have data about splicing and the axon too,,even in uniprot they have data of aa sequence but still unreviewed...