Hi Agnes

I should advice you to use the "Maniatis" protocol.

Standard Washing:

- wash 2x for 15 min in 50 ml of 2x SSC, 0.1 % SDS at room

temperature

- wash 2x for 15 min in 50 ml of 0.5x SSC, 0.1 % SDS at 55 ̊C

(prewarmed)

or

for higher stringency:

wash 2x for 15 min in 50 ml of 0.1x SSC, 0.1 % SDS at 60 ̊C

(prewarmed)

Hope it helps

Best

Paco

Thank you Francisco!