Ágnes Mosolygó-Lukács

4 Questions 6 Answers 0 Followers

Questions related from Ágnes Mosolygó-Lukács

Could anyone give me a step-by-step FISH protocol for Arabidopsis?

I have never performed FISH experiment on Arabidopsis.. I need a detailed protocol.

12 December 2018 3,537 2 View

How to wash Hybond N+ membrane (nylon) for DNA Dot Blot?

I have performed Dot Blot assay, but the background was too high. For washing the membrane PBS-Tween was used. Can anybody suggest other washing buffer?

07 July 2018 8,645 2 View

How can I avoid "ring signal" in Dot Blot?

When I performed Dot blot assay I always see a "ring signal" in the sonicated DNA sample (positive ctrl). What do you think, how can I avoid this phenomenon?

04 April 2018 9,931 6 View

Does anyone have experience with SYBR Gold DNA staining in Arabidopsis?

I will perform immuofluorescent labelling in Arabidopsis. For DNA stainig I can use SYBR Gold (there is no UV laser in the microscope). Does anyone have experience with SYBR Gold in fixed...

01 January 2017 834 0 View