Could anyone give me a step-by-step FISH protocol for Arabidopsis?

More Ágnes Mosolygó-Lukács's questions See All

How to wash Hybond N+ membrane (nylon) for DNA Dot Blot?

I have performed Dot Blot assay, but the background was too high. For washing the membrane PBS-Tween was used. Can anybody suggest other washing buffer?

06 July 2018 8,575 2 View

How can I avoid "ring signal" in Dot Blot?

When I performed Dot blot assay I always see a "ring signal" in the sonicated DNA sample (positive ctrl). What do you think, how can I avoid this phenomenon?

03 April 2018 9,886 6 View

Does anyone have experience with SYBR Gold DNA staining in Arabidopsis?

I will perform immuofluorescent labelling in Arabidopsis. For DNA stainig I can use SYBR Gold (there is no UV laser in the microscope). Does anyone have experience with SYBR Gold in fixed...

31 December 2016 771 0 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Good morning research fellows, Has anyone worked with senna alata plant extract on any kind of fish?

Literatures on the piscicidal effect of sennaa alata is scanty. if you have any related litrature either in the bark, leaves or roots used as treatment to any aquatic organism please kindly help...

28 February 2021 9,138 2 View

Experimental Design: How deal with pseudreplicates with immunological and clinical-chemical paramaeters?

Hello everybody, I am investigating the influence of a toxinon various clinical-chemical and haematological parameters in fish. I have three treatments (control, low dose, high dose) in my...

24 February 2021 1,243 1 View

Hi i want to ask that in order to find out LC 50 (96 hours) of particular toxin for fish. Should we keep the animals with food or without food?

if we expose the fish after acclimatization to particular toxin in order to find out LC 50 (96 hours).Should we keep the fish off feed or with feed for that duration. Some literature show that...

24 February 2021 357 2 View

Measuring Total immunoglobulin whole-body of fish larvae?

Hi Dear All, I prepared the extract of fish larvae whole body with PBS. Then protein concentration of the whole-body extraction samples determined by the Folin-Ceiocalteu-Phenol method (Lowry...

17 February 2021 1,971 3 View

What is the identity of additional bands found during fish gut microbiome 16S V4 PCR amplification?

Hi, I am carrying out test PCRs for 16S amplicon sequencing on Illumina MiSeq. I am using standard V4 primers: 515F/806R. The aim is to profile the microbiome of fish guts under different...

16 February 2021 5,668 6 View

Is this the fragment of a sauropod tooth?

Dear colleagues. Fragments of this species are very rare from a terrestrial Lower Cretaceous locality from Germany. Up to now from this locality sauropods could not be proved with certainty. Other...

14 February 2021 2,310 1 View

Problem to dissolve 50%wt Glycerol in 4%wt PVOH 28-99 ??

Hello everyone, I am trying to prepare a solution out of PVA(Polyvinyl alcohol)_Glycerol_Wasser . (4% PVA 28-99 and 50% Glycerin) To prepare this solution I first add PVA to 80degree water and...

06 February 2021 2,300 3 View

How to remove inter-year variation from my assessment of seasonal variation?

I have a 30 year dataset of fish sampling. I know from previous analyses that there is a huge amount of variation between years in catch rates. What I would like to do is create a plot that shows...

01 February 2021 3,980 5 View

Can anyone help to find any research articles related to the below mentioned objective?

Astaxanthin used in surimi based products as colour enhancer or as an antioxidant! Regards & Thanks Asik Ikbal

25 January 2021 9,399 4 View

Frederic Lepretre

Hi Agnes

there is a google book describing what you're searching for:

https://books.google.fr/books?id=TnDH-UvwhTAC&pg=PA173&lpg=PA173&dq=arabidopsis+FISH&source=bl&ots=vB7KltC_iz&sig=bDbA4CGpK2VKoJo8GiEtmKCFqS0&hl=fr&sa=X&ved=2ahUKEwjax86z2rrfAhVLaBoKHf2BClcQ6AEwCnoECFgQAQ#v=onepage&q=arabidopsis%20FISH&f=false

all the best

fred