Could anyone give me a step-by-step FISH protocol for Arabidopsis?

More Ágnes Mosolygó-Lukács's questions See All

How to wash Hybond N+ membrane (nylon) for DNA Dot Blot?

I have performed Dot Blot assay, but the background was too high. For washing the membrane PBS-Tween was used. Can anybody suggest other washing buffer?

06 July 2018 8,679 2 View

How can I avoid "ring signal" in Dot Blot?

When I performed Dot blot assay I always see a "ring signal" in the sonicated DNA sample (positive ctrl). What do you think, how can I avoid this phenomenon?

03 April 2018 9,953 6 View

Does anyone have experience with SYBR Gold DNA staining in Arabidopsis?

I will perform immuofluorescent labelling in Arabidopsis. For DNA stainig I can use SYBR Gold (there is no UV laser in the microscope). Does anyone have experience with SYBR Gold in fixed...

31 December 2016 855 0 View

How to quantify polystyrene microplastic (8 micron) bioaccumulation in fish tissue?

"I have treated adult zebrafish with 8-micron polystyrene microplastic and want to study the bioaccumulation in different organs. Can this be done using hydrogen peroxide digestion followed by...

05 August 2024 853 3 View

Do anyone knows any book dealing with fish growth/zootechnical performances and somatic indexes in aquaculture?

In the aquaculture and fish nutrition research field, a number of growth and somatic indexes are often used, including specific growth rate (SGR), feed conversion rate (FCR), protein efficiency...

22 July 2024 2,480 4 View

Why do fish have fishy odors naturally ?

Chemical Engineering /Food Engineering

20 July 2024 4,152 4 View

Chromosomal spreads are shrunken after fluorescence in situ hybridization (FISH), why?

Dear colleagues, We are trying to do FISH on mice PDEC metaphase spreads. We use homemade biotin labelled probes, synthetized using a nick translation kit. For hybridization, initially we were...

08 July 2024 8,687 3 View

Determination of fatty acids in fish?

I measured the fatty acids in the fish, using the internal standard method in the national standard method, I first extracted the fish oil with cable extraction, please ask me how many grams of...

02 July 2024 900 1 View

How many fish otoliths and charophyte gyrogonites should you find in a sedimentary sample weighing 50g in order to be considered enviromental indices?

It's for my thesis on Quaternary costal sediments from Western Greece.

01 July 2024 4,854 0 View

Does anyone knows about any protandrous fish inhabiting the Mediterranean Sea, smaller than 20 cm (adult) and that change sex relatively quickly?

Other than Sparids

27 June 2024 1,809 0 View

Fish physiology and biochemistry journal ?

No response from fish physiology and biochemistry journal after a year of peer review. Journal editorial team is not responding even for Request for withdrawal of our manuscript. We are struck...

25 June 2024 8,918 0 View

How to properly label fish feed pellets with stable isotopes for tracing C and N flow?

Hi, I´d like to trace the C and N flow in a recirculating aquaculture system by labelling the fish feed with concentrated stable isotopes. From a supplier, I found a product ouf of algae lysate...

19 June 2024 746 0 View

What are those vacuolated structures in my tilapia intestinal cross-sections (H&E stained)? Do the sections exhibit signs of enteritis?

Could someone please confirm if those vacuolated structures are goblet cells? And whether the sections exhibit signs of inflammation. Context: I am conducting histological analysis of the...

15 June 2024 3,208 4 View

Frederic Lepretre

Hi Agnes

there is a google book describing what you're searching for:

https://books.google.fr/books?id=TnDH-UvwhTAC&pg=PA173&lpg=PA173&dq=arabidopsis+FISH&source=bl&ots=vB7KltC_iz&sig=bDbA4CGpK2VKoJo8GiEtmKCFqS0&hl=fr&sa=X&ved=2ahUKEwjax86z2rrfAhVLaBoKHf2BClcQ6AEwCnoECFgQAQ#v=onepage&q=arabidopsis%20FISH&f=false

all the best

fred

Ágnes Mosolygó-Lukács

Thank you Frederic!