How to use rice cell culture for gene expression studies?

More Muhammad Naveed's questions See All

I have a query regarding journals payments in form of open access ?

As from last few months most of the journals are going toward citation and h-index and offering authors to pay fee in form open access option. Does it is justice with authors to pay money to...

08 September 2016 2,380 3 View

Dear Researchers : Any one can suggest me a tutorial or research articles on interpretation of Patch-dock (online Ligand-protein docking software) ?

Guide me any material on patch dock results interpretation. As well as any other easy to use and authentic online tool for Protein docking ?

31 December 2015 3,432 7 View

Hi, any one can share the latest and free link to download the Auto-dock vina for enzyme and ligand docking ?

please share some links for video tutorial on Auto-dock vina for hand on skill in protein docking

10 November 2015 1,172 2 View

What is suitable and easy to use protein docking tool?

Actually I would like to know the Ligand-protein interaction and to locate the exact position of co-enzyme in active site of the protein. Can you guide me to find out a freely available...

02 March 2015 4,618 13 View

How can a protein sequence be annotated/generated from the Gene sequence for GenBank submission?

From a DNA sequence, how can one confirm/make a protein sequence is correct (frame shift error, correct ORF strand and position ?) for sequence submission in GenBank. For Example DNA...

31 December 2014 5,144 21 View

How can I create a feature table for annotation of sequence submission in NCBI?

Actually I would like to correct/edit my sequences which are already submitted to NCBI GenBank database and GenBank team said to do these: for sequence updates 1. New sequence in FASTA format 2....

31 December 2014 7,436 10 View

What is the effectiveness of glycerol to preserve bacterial cultures at -80C ?

I want to ask, for how many time (years), a bacterial culture remain preserved with 40% glycerol at -80C ?. In the other sense if a culture remain in glycerol for 2 years then it have any effect...

11 December 2014 646 55 View

Would you recommend Multilocus sequence analysis (MLSA) or DNA-DNA hybridization?

What is the best molecular method to identify the novel bacterial specie, if we can use MLSA as a replacement of DNA-DNA Hybridization for this regard? Please post your suggestion here.

03 April 2014 2,744 12 View

What is the secret of sweetenss in carbohydrates?

What is the mechanism which makes carbohydrates taste sweet to our taste buds??

01 February 2014 7,437 6 View

Can someone recommend bioinformatics tools for protein novel insights, interaction, structure, function?

What kind of tools would you recommend to characterize a proteins for a specific trait, i have a pdb file of a GDH protein want to characterized it, which kind of the things to be characterized...

06 July 2013 4,236 11 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Microtome tissue sectioning - How to prevent chatter of paraffin tissue specimen sections?

Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...

06 August 2024 666 4 View

How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?

06 August 2024 9,618 3 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

Why did the authors extrapolate a phenotype that they experimentally proved in one bacterial strain across the whole genus of the organism?

I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...

05 August 2024 6,787 4 View

Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

05 August 2024 2,570 3 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

05 August 2024 7,727 4 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

Rajaguru Bohar

Good question looking forward for the replies, ill also search literatures related to it

Marcin Nowicki

http://scholar.google.pl/scholar?q=%22rice+cell+culture%22+expression&btnG=&hl=pl&as_sdt=0%2C5

Muhammad Naveed

thanks Marcin for sharing link but i already googled it so difficulty to find out a precise answer of above queries from google