Emily Tsui

6 Questions 5 Answers 0 Followers

Questions related from Emily Tsui

In cellular fractionation, do I need to sonicate every fraction?

Hi I would like to ask if I need to sonicate even the input, cytoplasm fraction and nuclear fraction before i boil and load my sample in western blot?

02 September 2022 4,354 0 View

How much HEPES should I add and how long can it last until I change culture medium?

I would like to add HEPES into my cell culture medium as I have an incubator but without CO2 supply, how much should I add and how long can it last until I change the medium again?

01 September 2020 3,670 4 View

Do I need to add serum if my cell culture medium already has HEPES?

Should I still add 10 % Calf serum if I supplement the cell culture medium with 25mM HEPES (for incubation without CO2)? I want to add HEPES as I dont have CO2 incubator My culture medium...

28 August 2020 7,663 11 View

How to do metaphase spread IF staining?

Pls help! I would like to do IF staining with 2 antibodies on a metaphase spread. But I dont have a cytospin! How to do it without cytospin? And im using methanol:acetic acid fixation.

17 August 2020 656 1 View

How to reverse cytochalasin B block of cytokinesis?

I would like to use it to detect micronuclei after ionizing radiation but after I identify the cells with them, I would want to let them continuously grow, can I just wash away the cytochalasinB?

19 June 2020 9,627 0 View

What does crest staining do?

I want to detect whether there's kinetochore formation on a possibly neocentromere, doest crest staining contain anti-kinetochore antibody and allow me to do that? Can someone suggest me a good...

01 December 2019 2,264 3 View