Emily Tsui

10 Questions 6 Answers 0 Followers

Questions related from Emily Tsui

In cellular fractionation, do I need to sonicate every fraction?

Hi I would like to ask if I need to sonicate even the input, cytoplasm fraction and nuclear fraction before i boil and load my sample in western blot?

02 September 2022 4,363 0 View

Any protein that localize to nucleus but absent from both chromosome and nucleolus?

Hi I'm studying a novel protein that when tagged observe localization in nucleus specifically but excluded from chromosome or nucleolus, any similar protein that has such localization? Or is there...

17 August 2021 4,068 2 View

How much HEPES should I add and how long can it last until I change culture medium?

I would like to add HEPES into my cell culture medium as I have an incubator but without CO2 supply, how much should I add and how long can it last until I change the medium again?

01 September 2020 3,682 4 View

Do I need to add serum if my cell culture medium already has HEPES?

Should I still add 10 % Calf serum if I supplement the cell culture medium with 25mM HEPES (for incubation without CO2)? I want to add HEPES as I dont have CO2 incubator My culture medium...

28 August 2020 7,695 11 View

How to do metaphase spread IF staining?

Pls help! I would like to do IF staining with 2 antibodies on a metaphase spread. But I dont have a cytospin! How to do it without cytospin? And im using methanol:acetic acid fixation.

17 August 2020 683 1 View

How to reverse cytochalasin B block of cytokinesis?

I would like to use it to detect micronuclei after ionizing radiation but after I identify the cells with them, I would want to let them continuously grow, can I just wash away the cytochalasinB?

19 June 2020 9,633 0 View

Why only binuclear cells are chosen for micronuclei assay?

Why studies choose binuclear cells with MN and do the assay without synchronizing the cells to methaphase?

08 June 2020 7,384 0 View

How to detect chromothripsis?

Is it possible to detect chromothripsis in micronuclei just by y-H2AX?

05 June 2020 6,106 0 View

After microirradiation, what temperature I should use for incubating HeLa & U2OS cells before fixation?

I've seen a study using room temperature but normally other articles use 37C cell incubator to store and let cells repair their damage and fix them.

18 March 2020 4,749 2 View

What does crest staining do?

I want to detect whether there's kinetochore formation on a possibly neocentromere, doest crest staining contain anti-kinetochore antibody and allow me to do that? Can someone suggest me a good...

01 December 2019 2,275 3 View