I want to clone this cDNA and over express them in MSCs can it be possible to amplify the CDS with gene specific primers? or is there any other procedure to amplify from tissue specific whole RNA?
If these genes are tissue-specific expression, isolate total RNA or mRNAs from the specific tissues for making cDNA. Make cDNA with two steps as mentioned by Andrey.
You can also do it in one step ( in the same reaction cDNA creation from mRNA and gene amplification using a specific primer) this will protect your product from contamination.