In regard to the preparation of acute brain slices, animals are anaesthetised, confirmed areflexia, and then decapitated. In my case, if I am going to study the hippocampus, then only the regions containing the hippocampus will be prepared as acute brain slices while other parts of the brain (e.g. cerebellum) and the rest of the body will be discarded.
As I think it is so wasteful that those precious discarded parts/tissues/cells can actually be reused in other studies/applications (e.g. make primary cells) especially my animals are not transgenic, I was wondering whether there is a way to preserve this parts. The ultimate storage will be in the -80C freezer.
Thank you very much in advance.
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Another issue, if I am going to recycle the discarded brain parts by making them as primary cells (neuron + glia co-culture), then what would be the best protocol for this purpose please?
Thank you very much in advance.
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