Are there any ways to analysis the muscle cross sectional area automatically?

Dear colleagues, I was wondering whether there are any ways or any softwares that I can use to analysis the muscle cross sectional area in my H&E histology images? I tried to use ImageJ...

03 April 2016 6,779 9 View

Potassium channel or Chloride channel?

If I am going to make a guess between the potassium channel and the chlroide channel by the following evidence: "hyperpolarization of pyramidal neurons and suppresses action potential firing in...

09 October 2015 552 0 View

What is the intracellular distribution of Matrix Metalloproteinase (MMP)-9 in fast motor neurons?

What is the intracellular distribution of Matrix Metalloproteinase (MMP)-9 in fast motor neurons (if possible, which organelle)? Do they found in the axon terminal? Additionally, do microglia...

08 September 2015 6,544 3 View

What is the prefered/best guage of Hamilton needle used for mice brain injections?

I am recently planning to do some stereotaxic injections to some mice brain nuclei. Due to the various choices (in particular the G number) of Hamilton needles, I was wondering which one would be...

02 March 2015 5,784 4 View

How to find out the best weight of my animal model when screening animals for experiment?

In the literature, there is often a description saying that ''an animal weight X to Y grams/pounds was used''. I was wondering how do the author know the best weight range of their experimental...

01 February 2015 2,374 7 View

What would be the injection coordinate for MFB in mice?

I am planning to do a stereotaxic injection to the medial forebrain bundle (MFB) of mice. I have tried AP:-1.2; ML:-1.1; DV:-5.0mm, but this does not seem to work. I was wondering whether there is...

01 February 2015 9,857 7 View

About controls, between individual or hemisphere?

I have a question regarding the use of control as follows: Although it is quite usual to setup 2 groups of animals (one for experiment, and the other for control), there is some paper/studies...

01 February 2015 2,805 8 View

How can I distinguish M1 and M2 in mice?

As M1 and M2 lie very close to each other, if I am going to do an implant to gather signals from only M1 neurons, is there any established way for me to locate accurately the M1 or M2? The...

31 December 2014 5,472 1 View

Why do we still need to use salines after fixing in histochemistries?

After the fixation with 4% paraformaldehyde (PFA), I assume that everything that is fixable by PFA will be preserved while those did not will lost much of the attention from the investigator (may...

31 December 2014 7,671 6 View

Is there a way to confirm the identity of the mouse M1 neuron?

If I am going to do an in vivo patch clamp on mice M1 neuron (primary motor cortex) only, is there a way to identify/confirm the area? To the best of my knowledge, M1 is close to M2 and these 2...

31 December 2014 5,493 4 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

Irradiation data for JAPAN?

Hi, Could you help me to get the latest (2019, 2020) solar resource data for a particular site in JAPAN? PV GIS and NSRDB data are not in reach to JAPAN, I'm looking for some other database for...

03 March 2021 961 3 View

No title

02 March 2021 3,060 3 View