I am recently planning to do some stereotaxic injections to some mice brain nuclei. Due to the various choices (in particular the G number) of Hamilton needles, I was wondering which one would be the best for my surgery.
If available, may I also have the "cat no" please? (I am thinking of purchasing one)
Thank you very much in advance once again!
For our orthotopic brain tumor brachytherapy model, we used a pt.style #3 Hamilton microsyringe. We choose this one since it fitted well trough our in-house made stereotactic screw with a central hole that fitted both the needle to administer tumour cells (week 1) , the iodine-125 brachytherapy seed (week 2) and/or a radiosensitizing drug.
see: Verhoef et al. Radiat Oncol 2007; 2(38) http://www.ro-journal.com/content/2/1/38
What is the aim?
Lesion studies, large (like 32G or 33G) might be fine,
looking at fine scale cell morphology in the target region everything below 34G is too large. Blunt needles are prone to clogging, Bvld more difficult to calculate the injection depth.
For many applications a glass capillary is equally well suited or can even be better, since you can work with an even much smaller diameter.
Thank you all for the suggestions and information. Actually, I am trying to inject 6-OHDA into the medial forebrain bundle (MFB) of the mice to create a PD model.
My case is: I tried my lab mate's needle which is commonly used for rat injections (with many successful experiences). However, on mice, there is no success case so far.
We are current using the 7-week-old mice (seems like age of the animal is also an important factor as it may affect the injection coordinates).
what is the maximum volume that can be injected into a rat brain stereotypically?
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